SPINK4 Protein (His Tag)

Serine Protease Inhibitor Kazal-type 4 Protein

SPINK4 Protein Product Information

• Synonym: SPINK4, MGC133107, PEC-60
• Protein Construction: A DNA sequence encoding the human SPINK4 (NP_055286.1) (Met 1-Cys 86) was expressed, fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Human Cells

SPINK4 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gly 27
• Molecular Mass: The recombinant human SPINK4 consists of 71 amino acids and has a predicted molecular mass of 8 KDa. It migrates as an approximately 10 KDa band in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
• SDS-PAGE: SPINK4 protein

SPINK4 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SPINK4 Protein Description

Serine protease inhibitor Kazal-type 4, also known as Peptide PEC-60 homolog and SPINK4, is a secreted protein which contains one Kazal-like domain. SPINK4 is a member of the SPINK protein family. The gene family of serine protease inhibitors of the Kazal type (SPINK) are functional and positional candidate genes for celiac disease (CD). SPINK1 plays an important role in protecting the pancreas against excessive trypsinogen activation. It is a potent natural inhibitor of pancreatic trypsin activity. SPINK1 mutations are associated with the development of acute and chronic pancreatitis and have been detected in all forms of chronic pancreatitis. SPINK2 functions as a trypsin/acrosin inhibitor and is synthesized mainly in the testis and seminal vesicle where its activity is engaged in fertility. The SPINK2 protein contains a typical Kazal domain composed by six cysteine residues forming three disulfide bridges. SPINK9 was identified in human skin. Its expression was strong in palmar epidermis, but not detectable or very low in non palmoplantar skin.

References

1. Schneider, A. et al., 2004,Gastroenterol Clin North Am 33 (4): 789-806.
2. Wapenaar, MC. et al., 2007, Immunogenetics  59 (5): 349-57.
3. Brattsand, M. et al., 2009, J Invest Dermatol 129 (7): 1656-65.
4. Chen, T. et al., 2009, Proteins. 77 (1): 209-19.
5. Noah, TK. et al., 2010, Exp Cell Res. 316 (3): 452-65.