SPINK4 Antibody (Antigen Affinity Purified)

Serine Protease Inhibitor Kazal-type 4 Antibody ( Antigen Affinity Purified )

SPINK4 Antibody Product Information

• Immunogen: Recombinant human SPINK4 protein ( Catalog#11669-H08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human SPINK4 ( rh SPINK4 ; Catalog#11669-H08H ; NP_055286.1 ; Met 1 - Cys 86 ). SPINK4 specific IgG was purified by human SPINK4 affinity chromatography

SPINK4 Antibody Usage Guide

• Specificity: Human SPINK4
• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human SPINK4 in WB. Using a DAB detection system, the detection limit for human SPINK4 is approximately 4 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human SPINK4. The detection limit for human SPINK4 is approximately 0.00975 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

SPINK4 Antibody Background

Serine protease inhibitor Kazal-type 4, also known as Peptide PEC-60 homolog and SPINK4, is a secreted protein which contains one Kazal-like domain. SPINK4 is a member of the SPINK protein family. The gene family of serine protease inhibitors of the Kazal type (SPINK) are functional and positional candidate genes for celiac disease (CD). SPINK1 plays an important role in protecting the pancreas against excessive trypsinogen activation. It is a potent natural inhibitor of pancreatic trypsin activity. SPINK1 mutations are associated with the development of acute and chronic pancreatitis and have been detected in all forms of chronic pancreatitis. SPINK2 functions as a trypsin/acrosin inhibitor and is synthesized mainly in the testis and seminal vesicle where its activity is engaged in fertility. The SPINK2 protein contains a typical Kazal domain composed by six cysteine residues forming three disulfide bridges. SPINK9 was identified in human skin. Its expression was strong in palmar epidermis, but not detectable or very low in non palmoplantar skin.

References

1. Schneider, A. et al., 2004,Gastroenterol Clin North Am 33 (4): 789-806.
2. Wapenaar, MC. et al., 2007, Immunogenetics  59 (5): 349-57.
3. Brattsand, M. et al., 2009, J Invest Dermatol 129 (7): 1656-65.
4. Chen, T. et al., 2009, Proteins. 77 (1): 209-19.
5. Noah, TK. et al., 2010, Exp Cell Res. 316 (3): 452-65.