Human SPINK1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13357-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

SPINK1 cDNA Clone Datasheet

SPINK1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC025790
cDNA Size:	240
Gene Synonym:	PCTT; PSTI; TATI; Spink3; SPINK1
Source:	Human

SPINK1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens serine peptidase inhibitor, Kazal type 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

SPINK1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SPINK1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SPINK1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGGTAA CAGGCATCTT TCTTCTCAGT GCCTTGGCCC TGTTGAGTCT ATCTGGTAAC
61       ACTGGAGCTG ACTCCCTGGG AAGAGAGGCC AAATGTTACA ATGAACTTAA TGGATGCACC
121      AAGATATATG ACCCTGTCTG TGGGACTGAT GGAAATACTT ATCCCAATGA ATGCGTGTTA
181      TGTTTTGAAA ATCGGAAACG CCAGACTTCT ATCCTCATTC AAAAATCTGG GCCTTGCTGA

Quality Control:cDNA sequencing results is available upon request.