Mouse ATL1 Gene cDNA Clone / ORF Clone

ATL1 cDNA Clone Datasheet

ATL1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_178628.5
• cDNA Size: 1677
• Gene Synonym: Fsp1; Spg3; Adfsp; Spg3a; 4930435M24Rik; Atl1
• Source: Mouse

ATL1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus atlastin GTPase 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 204 T/C not causing the amino acid variation.

ATL1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ATL1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ATL1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTAAGA GCCGCAGGGA CAGAAACAGT TGGGGTGGGT TTTCAGAGAA GTCAAGTGAC
61       TGGAGCTCAG AAGAGGAGGA GCCAGTGAGA AAGGCAGGGC CTGTCCAGGT CCTCATCGTC
121      AAGGACGACC ATTCCTTTGA GTTAGACGAG GCTGCGCTGA ACCGCATCCT TCTGTCCCAG
181      GCCGTCAGAG ACAAGGAGGT GGTCGCTGTG TCTGTTGCTG GAGCCTTTAG AAAAGGGAAA
241      TCCTTCCTGA TGGACTTCAT GTTGCGGTAC ATGTACAACC AGGAATCTGT TGATTGGGTT
301      GGAGATTACA ATGAACCATT GACTGGATTT TCATGGAGGG GTGGATCGGA ACGAGAAACC
361      ACAGGAATCC AGATATGGAG TGAAGTTTTC CTCATCAATA AGCTTGATGG TAAAAAGGTT
421      GCAGTGTTAC TGATGGATAC TCAGGGAACC TTTGACAGTC AGTCAACTTT GAGAGATTCG
481      GCCACAGTAT TTGCGCTTAG CACAATGATA AGCTCAATAC AGGTCTACAA CTTGTCCCAG
541      AATGTCCAGG AGGATGATCT CCAGCACCTG CAGCTTTTCA CTGAGTATGG CAGGTTGGCA
601      ATGGAAGAAA CATTCCTGAA GCCTTTTCAG AGTCTGATAT TTCTTGTTCG AGACTGGAGT
661      TTCCCATACG AATTTTCATA TGGAGCTGAT GGTGGCGCCA AGTTCTTGGA AAAACGCCTC
721      AAGGTCTCAG GGAACCAGCA TGAAGAGCTG CAGAACGTCA GGAAGCACAT CCACTCCTGC
781      TTCACCAACA TTTCCTGCTT TCTCCTACCT CATCCTGGCT TAAAAGTCGC TACCAACCCA
841      AACTTTGATG GGAAACTAAA AGAAATAGAT GATGAATTCA TCAAAAACTT GAAAATACTG
901      ATTCCATGGC TCCTGAGTCC TGAGCGTCTA GATATTAAAG AGATCAATGG GAATAAAATC
961      ACCTGCCGAG GCCTGCTGGA GTACTTCAAG GCATACATAA AGATATACCA AGGTGAAGAA
1021     TTGCCGCATC CCAAGTCCAT GTTACAGGCC ACAGCAGAAG CTAACAATTT AGCAGCTGTT
1081     GCAACTGCCA AGGACACATA CAACAAGAAG ATGGAAGAGG TTTGTGGTGG TGATAAACCA
1141     TTTTTGGCCC CTAACGACCT GCAAAGCAAG CACCTGCAGC TAAAAGAAGA ATCTGTGAAG
1201     TTATTCCGAG GCGTGAAGAA GATGGGCGGG GAAGAGTTCA GCCGGCGTTA CCTGCAGCAG
1261     CTGGAGAGTG AAATAGATGA ACTCTACATC CAGTATATTA AGCACAATGA CAGCAAAAAT
1321     ATCTTCCATG CTGCTCGAAC CCCAGCCACA CTGTTCGTTG TCATCTTTAT CACATACGTG
1381     ATTGCTGGTG TGACTGGATT CATTGGTTTG GACATCATAG CTAGCCTGTG CAACATGATC
1441     ATGGGACTGA CGCTTATCAC CCTCTGCACC TGGGCATATA TCCGCTACTC TGGCGAATAT
1501     CGAGAGCTGG GTGCTGTAAT CGACCAGGTA GCTGCAGCTT TGTGGGACCA GGGAAGCACA
1561     AATGAGGCGT TATACAAGCT TTACAGTGCA GCAGCAACCC ACAGACACCT GTGTCACCAA
1621     GCTTTCCCTG CACCCAAGTC AGAGCCTACT CAACAACCGG AAAAGAAGAA AATTTAA

 

Quality Control:cDNA sequencing results is available upon request.