APEG1 cDNA ORF Clone, Human, untagged General Information
Identical with the Gene Bank Ref. ID sequence.
Full length Clone DNA of Human SPEG complex locus.
Enhanced CMV promoter
KpnI + XhoI (5.5kb + 0.34kb)
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
The plasmid is confirmed by full-length sequencing.
Antibiotic in E.coli
Antibiotic in Mammalian cell
Stable or Transient mammalian expression
Storage & Shipping
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
APEG1 cDNA ORF Clone, Human, untagged Validated Images
APEG1 cDNA ORF Clone, Human, untagged Alternative Names
APEG-1 cDNA ORF Clone, Human;APEG1 cDNA ORF Clone, Human;BPEG cDNA ORF Clone, Human;CNM5 cDNA ORF Clone, Human;SPEGalpha cDNA ORF Clone, Human;SPEGbeta cDNA ORF Clone, Human
APEG1 Background Information
Striated muscle preferentially expressed protein kinase, also known as aortic preferentially expressed protein 1, APEG-1, SPEG and KIAA1297, is a protein which belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. SPEG / APEG-1 contains two fibronectin type-III domains, nine Ig-like (immunoglobulin-like) domains, two protein kinase domains. Isoform 1 of SPEG is preferentially expressed in striated muscle. Non-kinase form such as isoform 3 of SPEG is predominantly expressed in the aorta. Isoform 3 of SPEG appears to be expressed only in highly differentiated ASMC in normal vessel walls and down-regulated in dedifferentiated ASMC. Isoform 3 of SPEG may have a role in regulating the growth and differentiation of arterial smooth muscle cells. Isoform 3 of SPEG is quickly down-regulated in response to vascular injury, when ASMC cells change from a quiescent to a proliferative phenotype.
SPEG complex locus
Hsieh C.-M., et al., 1996, J. Biol. Chem. 271:17354-17359. Manjasetty B.A., et al., 2005, BMC Struct. Biol. 5:21-21. Zhou,Y. et al., 2007, Anal Chem. 79 (15): 5826-37. Greenman C., et al., 2007, Nature. 446:153-158.