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SPARCL1 / MAST9 Antibody, Mouse MAb

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Human SPARCL1 Antibody Product Information
Immunogen:Recombinant Human SPARCL1 protein (Catalog#10046-H08H)
Clone ID:6D1D6G5
Ig Type:Mouse IgG1
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SPARCL1 (rhSPARCL1; Catalog#10046-H08H; NP_004675.3; Met 1-Phe 664). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human SPARCL1 Antibody Usage Guide
Specificity:Human SPARCL1 / MAST9
No cross-reactivity with Human cell lysate (293 cell line) in WB and ELISA.
Application:ELISA, ICC/IF, IF, FCM

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human SPARCL1. The detection limit for SPARCL1 is 0.0195 ng/well.

FCM: 0.5-2 μg/Test

ICC/IF: 10-25 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human SPARCL1 Antibody IF Application Image
SPARCL1 / MAST9 Antibody, Mouse MAb, Immunofluorescence
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Immunofluorescence staining of Human SPARCL1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human SPARCL1 monoclonal antibody (15 µg/ml). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoplasm.
Human SPARCL1 Antibody FC Application Image
SPARCL1 / MAST9 Antibody, Mouse MAb, Flow cytometric analysis
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Flow cytometric analysis of Human SPARCL1 expression on HL60 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human SPARCL1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Other SPARCL1 Antibody Products
SPARCL1 / SPARC-like 1 Background

SPARC-like protein 1 (SPARCL1; also known as SC1, high endothelial venule protein, or hevin) is an extracellular matrix-associated, secreted glycoprotein belonging to the secreted protein acidic and rich in cysteine (SPARC) family of matricellular proteins. It contains three conserved structural domains that are implicated in the regulation of cell adhesion, migration, and proliferation. SPARCL1 is expressed during embryogenesis and tissue remodeling and is especially prominent in brain and vasculature. Its down-regulation in a number of cancers and the possibility of its functional compensation by SPARC has led to recent interest in hevin as a tumor suppressor and regulator of angiogenesis. SPARCL1 has antiadhesive properties, and loss of SPARCL1 expression is associated with increased proliferative activity and cell cycle progression. It is suggested that it may influence multiple cellular processes during distinct stages of brain development and function. In addition, SPARCL1 can influence the function of astroglial cells in the developing and mature central nervous system (CNS).

Human SPARCL1 / SPARC-like 1 References
  • Sullivan MM, et al. (2004) Hevin/SC1, a matricellular glycoprotein and potential tumor-suppressor of the SPARC/BM-40/Osteonectin family. Int J Biochem Cell Biol. 36(6): 991-6.
  • Esposito I, et al. (2007) Tumor-suppressor function of SPARC-like protein 1/Hevin in pancreatic cancer. Neoplasia. 9(1): 8-17.
  • Weimer JM, et al. (2008) A BAC transgenic mouse model to analyze the function of astroglial SPARCL1 (SC1) in the central nervous system. Glia. 56(9): 935-41.
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    Catalog: 10046-MM06-200
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