Anti-SPARCL1 Antibody

Cat: 10046-MM06
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Anti-SPARCL1 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-SPARCL1 Antibody
Validated applications
Species reactivity
Reacts with: Human
Recombinant Human SPARCL1 protein (Catalog#10046-H08H)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SPARCL1 (rhSPARCL1; Catalog#10046-H08H; NP_004675.3; Met 1-Phe 664). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #6D1D6G5
Protein A
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-SPARCL1 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
ICC-IF 1:20-1:100  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-SPARCL1 Antibody (Mouse Monoclonal antibody) Images
Human SPARCL1 / SPARC-like 1 Immunofluorescence(IF) 2857
Immunofluorescence staining of Human SPARCL1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human SPARCL1 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoplasm.
Human SPARCL1 / SPARC-like 1 Flow Cytometry (FC) 5025
Flow cytometric analysis of Human SPARCL1 expression on HL60 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human SPARCL1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Anti-SPARCL1 Antibody Alternative Names
Anti-MAST 9 Antibody;Anti-MAST9 Antibody;Anti-PIG33 Antibody;Anti-SC1 Antibody;Anti-SPARCL1 Antibody
SPARCL1 Background Information

SPARC-like protein 1 (SPARCL1; also known as SC1, high endothelial venule protein, or hevin) is an extracellular matrix-associated, secreted glycoprotein belonging to the secreted protein acidic and rich in cysteine (SPARC) family of matricellular proteins. It contains three conserved structural domains that are implicated in the regulation of cell adhesion, migration, and proliferation. SPARCL1 is expressed during embryogenesis and tissue remodeling and is especially prominent in brain and vasculature. Its down-regulation in a number of cancers and the possibility of its functional compensation by SPARC has led to recent interest in hevin as a tumor suppressor and regulator of angiogenesis. SPARCL1 has antiadhesive properties, and loss of SPARCL1 expression is associated with increased proliferative activity and cell cycle progression. It is suggested that it may influence multiple cellular processes during distinct stages of brain development and function. In addition, SPARCL1 can influence the function of astroglial cells in the developing and mature central nervous system (CNS).

Full Name
SPARC like 1
  • Sullivan MM, et al. (2004) Hevin/SC1, a matricellular glycoprotein and potential tumor-suppressor of the SPARC/BM-40/Osteonectin family. Int J Biochem Cell Biol. 36(6): 991-6.
  • Esposito I, et al. (2007) Tumor-suppressor function of SPARC-like protein 1/Hevin in pancreatic cancer. Neoplasia. 9(1): 8-17.
  • Weimer JM, et al. (2008) A BAC transgenic mouse model to analyze the function of astroglial SPARCL1 (SC1) in the central nervous system. Glia. 56(9): 935-41.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-SPARCL1 Antibody Rabbit Polyclonal ELISA 50544-RP01
    Anti-SPARCL1 Antibody Rabbit Polyclonal ELISA 50544-T16
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    Anti-SPARCL1 Antibody Rabbit Monoclonal ICC/IF,IF 10046-R390
    Anti-SPARCL1 Antibody (PE) Rabbit Monoclonal FCM 10046-R390-P
    Anti-SPARCL1 Antibody Mouse Monoclonal ELISA(Det),FCM,ICC/IF,IF 10046-MM05
    Anti-SPARCL1 Antibody (PE) Mouse Monoclonal FCM 10046-MM06-P
    Anti-SPARCL1 Antibody (FITC) Mouse Monoclonal FCM 10046-MM06-F
    Anti-SPARCL1 Antibody (FITC) Rabbit Monoclonal FCM 10046-R390-F
    Anti-SPARCL1 Antibody Rabbit Polyclonal ELISA 10046-RP01
    Anti-SPARCL1 Antibody Rabbit Polyclonal ELISA 10046-T16
    Anti-SPARCL1 Antibody Mouse Monoclonal ELISA(Cap) 10046-MM01
    Anti-SPARCL1 Antibody Rabbit Monoclonal ELISA 10046-R204
    Anti-SPARCL1 Antibody Rabbit Polyclonal ELISA,IHC-P 10046-T24
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