Anti-SOD2 Antibody

Cat: 12656-RP02
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Anti-SOD2 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-SOD2 Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human SOD2
Immunogen
Recombinant Human SOD2 protein (Catalog#12656-HNAE)
Preparation
Produced in rabbits immunized with purified, recombinant Human SOD2 (rh SOD2; Catalog#12656-HNAE; P04179-1; Lys 25-Lys 222). SOD2 specific IgG was purified by Human SOD2 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-SOD2 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IHC-P 1:500-1:2000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-SOD2 Antibody (Rabbit Polyclonal antibody) Images
Human SOD2 Immunohistochemistry(IHC) 3566
Immunochemical staining of human SOD2 in human placenta with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Human SOD2 Immunohistochemistry(IHC) 3568
Immunochemical staining of human SOD2 in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Human SOD2 Immunohistochemistry(IHC) 3571
Immunochemical staining of human SOD2 in human colon carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Human SOD1 Immunoprecipitation(IP) 8107

SOD1 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-SOD1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-SOD1 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 16 kDa

Observed band size: 16 kDa

Human SOD2 Western blot (WB) 13510

Anti-SOD2 rabbit polyclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:24 kDa

Observed band size:25 kDa

(We are unsure as to the identity of these extra bands.)

Anti-SOD2 Antibody Alternative Names
Anti-IPOB Antibody;Anti-MNSOD Antibody;Anti-MVCD6 Antibody
SOD2 Background Information

Superoxide dismutases (SOD) are important anti-oxidant enzymes that guard against superoxide toxicity. In humans, as in all mammals and most chordates, three forms of superoxide dismutase (SOD) are present: SOD1 is located in the cytoplasm, SOD2 in the mitochondria, and SOD3 is extracellular. Mitochondrial superoxide dismutase [SOD; manganese SOD (MnSOD) or SOD2] neutralizes highly reactive superoxide radical (O(

Full Name
superoxide dismutase 2, mitochondrial
References
  • Culotta VC, et al. (2006) Activation of superoxide dismutases: putting the metal to the pedal. Biochim Biophys Acta. 1763(7): 747-58.
  • Bag A, et al. (2008) Target sequence polymorphism of human manganese superoxide dismutase gene and its association with cancer risk: a review. Cancer Epidemiol Biomarkers Prev. 17(12): 3298-305.
  • Diehl C, et al. (2009) The basis of topical superoxide dismutase antipruritic activity. Acta Dermatovenerol Croat. 17(1): 25-39.
  • Ma X, et al. (2010) No association between SOD2 Val16Ala polymorphism and breast cancer susceptibility: a meta-analysis based on 9,710 cases and 11,041 controls. Breast Cancer Res Treat. 122(2): 509-14.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-SOD2 Antibody Rabbit Monoclonal WB,ELISA,IP 12656-R001
    Anti-SOD2 Antibody Rabbit Polyclonal ELISA 12656-RP01
    Anti-SOD2 Antibody Rabbit Monoclonal IHC-P 12656-R012
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