Anti-SOD2 Antibody

Cat: 12656-R001
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Anti-SOD2 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-SOD2 Antibody
Validated applications
Species reactivity
Reacts with: Human
Human SOD2
Recombinant Human SOD2 protein (Catalog#12656-HNAE)
This antibody was obtained from a rabbit immunized with purified, recombinant Human SOD2 (rh SOD2; Catalog#12656-HNAE; P04179-1; Lys 25-Lys 222).
Monoclonal Rabbit IgG Clone #001
Protein A
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-SOD2 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:2000-1:10000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-SOD2 Antibody (Rabbit Monoclonal antibody) Images
Human SOD2 Immunoprecipitation(IP) 6192

SOD2 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-SOD2 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-SOD2 rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 16 kDa

Observed band size: 18 kDa

Human SOD2 Western blot (WB) 13509

Anti-SOD2 rabbit monoclonal antibody at 1:10000 dilution

Lane A: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:25 kDa

Observed band size:25 kDa

Anti-SOD2 Antibody Alternative Names
Anti-IPOB Antibody;Anti-MNSOD Antibody;Anti-MVCD6 Antibody
SOD2 Background Information

Superoxide dismutases (SOD) are important anti-oxidant enzymes that guard against superoxide toxicity. In humans, as in all mammals and most chordates, three forms of superoxide dismutase (SOD) are present: SOD1 is located in the cytoplasm, SOD2 in the mitochondria, and SOD3 is extracellular. Mitochondrial superoxide dismutase [SOD; manganese SOD (MnSOD) or SOD2] neutralizes highly reactive superoxide radical (O(

Full Name
superoxide dismutase 2, mitochondrial
  • Culotta VC, et al. (2006) Activation of superoxide dismutases: putting the metal to the pedal. Biochim Biophys Acta. 1763(7): 747-58.
  • Bag A, et al. (2008) Target sequence polymorphism of human manganese superoxide dismutase gene and its association with cancer risk: a review. Cancer Epidemiol Biomarkers Prev. 17(12): 3298-305.
  • Diehl C, et al. (2009) The basis of topical superoxide dismutase antipruritic activity. Acta Dermatovenerol Croat. 17(1): 25-39.
  • Ma X, et al. (2010) No association between SOD2 Val16Ala polymorphism and breast cancer susceptibility: a meta-analysis based on 9,710 cases and 11,041 controls. Breast Cancer Res Treat. 122(2): 509-14.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-SOD2 Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 12656-RP02
    Anti-SOD2 Antibody Rabbit Polyclonal ELISA 12656-RP01
    Anti-SOD2 Antibody Rabbit Monoclonal IHC-P 12656-R012
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