- EGFR Signaling Pathway
- TGF-beta Signaling
- Canonical Wnt Signaling
- non-Canonical Wnt Signaling
- Notch Signaling
- p53 Pathway
- NF-kB Pathway
- Cytokine Signaling
|Catalog||Size (Price)||Quantity||In Stock||Operation|
SOD2 Antibody Datasheet
|Order or Inquire for SOD2 Antibody product||Quality antibodies||Antibody production services|
|Detection limit is 0. 039 ng/well in ELISA|
SOD2 Antibody Product Information
Recombinant Human SOD2 protein (Catalog#12656-HNAE)
|Antibody Type :||Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )|
Clone ID :
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.|
This antibody was obtained from a rabbit immunized with purified, recombinant Human SOD2 (rh SOD2; Catalog#12656-HNAE; P04179-1; Lys 25-Lys 222).
SOD2 Antibody Usage Guide
|Western blot :||
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human ALDH7A1 in WB.
|Direct ELISA :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SOD2. The detection limit for Human SOD2 is approximately 0. 039 ng/well.|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
SOD2 Antibody Related Products & Topics
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|SOD2||Human||SOD2 Protein, Recombinant, Native||12656-HNAE|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|Human SOD2||WB, ELISA||SOD2 Antibody (Antigen Affinity Purified)||12656-RP02|
|Human SOD2||ELISA||SOD2 Antibody||12656-R001|
SOD2 Antibody Background
Superoxide dismutases (SOD) are an ubiquitous family of enzymes that function to efficiently catalyze the dismutation of superoxide anions. Three unique and highly compartmentalized mammalian superoxide dismutases have been biochemically and molecularly characterized to date. SOD2, or Mn-SOD, exists as a tetramer and is initially synthesized containing a leader peptide, which targets this manganese-containing enzyme exclusively to the mitochondrial spaces. SOD2 is a member of the iron/manganese superoxide dismutase family. The SOD2 protein transforms toxic superoxide, a byproduct of the mitochondrial electron transport chain, into hydrogen peroxide and diatomic oxygen. In the case of mitochondrial MnSOD of eukaryotes (SOD2), metal insertion cannot occur post-translationally, but requires new synthesis and mitochondrial import of the SOD2 polypeptide. SOD2 can also bind iron in vivo, but is inactive with iron. Such metal ion mis-incorporation with SOD2 can become prevalent upon disruption of mitochondrial metal homeostasis. It is believed that oxidative stress plays an essential role in the development of breast cancer, while SOD2 is one of the primary enzymes that directly convert potential harmful oxidizing species to harmless metabolites. SOD2 Val16Ala polymorphism is not associated with breast cancer susceptibility.
- Zelko IN. et al., 2002, Free Radic Biol Med. 33 (3): 337-49.
- Culotta VC. et al., 2006, Biochim Biophys Acta. 1763 (7): 747-58.
- Ma X. et al., 2010, Breast Cancer Res Treat. 122 (2): 509-14.