Anti-SOD1 Antibody

Cat: 11727-MM11
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Anti-SOD1 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-SOD1 Antibody
Validated applications
WB,FCM,IP
Species reactivity
Reacts with: Human
Specificity
Human SOD1
Immunogen
Recombinant Human SOD1 / Superoxide Dismutase protein (Catalog#11727-H07E)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SOD1 / Superoxide Dismutase (rh SOD1 / Superoxide Dismutase; Catalog#11727-H07E; NP_000445.1; Ala 2-Gln 154).
Source
Monoclonal Mouse IgG2b Clone #11
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-SOD1 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
FCM 1:100-1:500  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-SOD1 Antibody (Mouse Monoclonal antibody) Images
Human SOD1 Flow Cytometry (FC) 5028
Flow cytometric analysis of Human SOD1 expression in HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-SOD1 (11727-MM11), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Human SOD1 Flow Cytometry (FC) 5144
Flow cytometric analysis of Human IDH1 expression in HepG2 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-IDH1 (12055-MM03), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Human ADAM12 Immunoprecipitation(IP) 8029

SOD1 was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

Lane B:0.5 mg HepG2 Whole Cell Lysate

0.5 µL anti-SOD1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-SOD1 mouse monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 16 kDa

Observed band size: 18 kDa

Human SOD1 Western blot (WB) 13443

Anti-SOD1 mouse monoclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lane C: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:16 kDa

Observed band size:20 kDa

Anti-SOD1 Antibody Alternative Names
Anti-ALS Antibody;Anti-ALS1 Antibody;Anti-HEL-S-44 Antibody;Anti-homodimer Antibody;Anti-hSod1 Antibody;Anti-IPOA Antibody;Anti-SOD Antibody
SOD1 Background Information

SOD1 belongs to the Cu-Zn superoxide dismutase family. It binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. SOD1 destroys radicals which are normally produced within the cells and which are toxic to biological systems. Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1). ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.

Full Name
superoxide dismutase 1, soluble
References
  • Murakami K, et al. (2011) SOD1 (copper/zinc superoxide dismutase) deficiency drives amyloid β protein oligomerization and memory loss in mouse model of Alzheimer disease. J Biol Chem. 286(52):44557-68.
  • Thompson M, et al. (2012) Paradoxical roles of serine racemase and D-serine in the G93A mSOD1 mouse model of amyotrophic lateral sclerosis. J Neurochem. 120(4):598-610.
  • Magrané J, et al. (2012) Mitochondrial dynamics and bioenergetic dysfunction is associated with synaptic alterations in mutant SOD1 motor neurons. J Neurosci. 32(1):229-42.
  • Gertz B, et al. (2012) Nuclear localization of human SOD1 and mutant SOD1-specific disruption of survival motor neuron protein complex in transgenic amyotrophic lateral sclerosis mice. J Neuropathol Exp Neurol. 71(2):162-77.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-SOD1 Antibody Rabbit Monoclonal ELISA,ICC/IF,IF 11727-R101
    Anti-SOD1 Antibody (PE) Mouse Monoclonal FCM 11727-MM11-P
    Anti-SOD1 Antibody Rabbit Monoclonal FCM 11727-R106
    Anti-SOD1 Antibody Mouse Monoclonal ELISA(Cap) 11727-MM10
    Anti-SOD1 Antibody Rabbit Polyclonal WB,ELISA,IP 11727-RP02
    Anti-SOD1 Antibody (FITC) Mouse Monoclonal FCM 11727-MM11-F
    Anti-SOD1 Antibody (FITC) Rabbit Monoclonal FCM 11727-R106-F
    Anti-SOD1 Antibody (PE) Rabbit Monoclonal FCM 11727-R106-P
    Anti-SOD1 Antibody Rabbit Polyclonal ELISA 11727-RP01
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