|IP||1-4 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
SOD1 was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate
Lane B:0.5 mg HepG2 Whole Cell Lysate2 µL anti-SOD1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-SOD1 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 16 kDa
Observed band size: 16 kDa
Anti-SOD1 rabbit polyclonal antibody at 1:500 dilution
Lane A: A431 Whole Cell Lysate
Lane B: HepG2 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:16 kDa
Observed band size:22 kDa
SOD1 belongs to the Cu-Zn superoxide dismutase family. It binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. SOD1 destroys radicals which are normally produced within the cells and which are toxic to biological systems. Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1). ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-SOD1 Antibody (PE)||Mouse||Monoclonal||FCM||11727-MM11-P|
|Anti-SOD1 Antibody (FITC)||Mouse||Monoclonal||FCM||11727-MM11-F|
|Anti-SOD1 Antibody (FITC)||Rabbit||Monoclonal||FCM||11727-R106-F|
|Anti-SOD1 Antibody (PE)||Rabbit||Monoclonal||FCM||11727-R106-P|