Anti-alpha-Synuclein Antibody

Cat: 12093-R017
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Anti-alpha-Synuclein Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-alpha-Synuclein Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human alpha-Synuclein
Immunogen
Recombinant Human SNCA / alpha-Synuclein protein (Catalog#12093-HNAE)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human SNCA / alpha-Synuclein (rh SNCA / alpha-Synuclein; Catalog#12093-HNAE; P37840-1; Met1-Ala140).
Source
Monoclonal Rabbit IgG Clone #017
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-alpha-Synuclein Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-alpha-Synuclein Antibody (Rabbit Monoclonal antibody) Images
Human SNCA/alpha-Synuclein Western blot (WB) 13467

Anti-SNCA rabbit monoclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:16 kDa

Observed band size:18 kDa

Human SNCA/alpha-Synuclein Immunoprecipitation(IP) 17699

SNCA was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

2 µL anti-SNCA rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-SNCA rabbit monoclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 16 kDa

Observed band size: 18 kDa

Anti-alpha-Synuclein Antibody Alternative Names
Anti-NACP Antibody;Anti-PARK1 Antibody;Anti-PARK4 Antibody;Anti-PD1 Antibody
alpha-Synuclein Background Information

Alpha-Synuclein (alpha-Syn), also known as NACP or SNCA, exists as at least two structural isoforms: one is helix-rich, membrane-bound form that both the N- and C-terminal regions of alpha-synuclein are tightly associated with membranes and the other is disordered, cytosolic form. Synuclein is found predominantly in the presynaptic termini, in both free or membrane-bound forms. SNCA is extensively localized in nucleus of neurons. It has been shown that alpha-Synuclein was highly expressed in the mitochondria in olfactory bulb, hippocampus, striatum, and thalamus, where the cytosolic alpha-Synuclein was also rich. Normally the unstructured soluble type of alpha-synuclein can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy bodies and multiple system atrophy. SNCA abnormality and mitochondrial deficiency are two major changes in the brain of patients with Parkinson's disease (PD). In addition, alpha-synuclein is an abundant component of Lewy bodies in sporadic Parkinson's disease and diffuse Lewy body disease.

Full Name
synuclein, alpha (non A4 component of amyloid precursor)
References
  • Arima K, et al. (1998) Immunoelectron-microscopic demonstration of NACP / alpha-synuclein-epitopes onthe filamentous component of Lewy bodies in Parkinson's disease and in dementia with Lewy bodies. Brain Res. 808 (1): 93-100.
  • Arima K, et al. (1998) NACP / alpha-synuclein immunoreactivity in fibrillary components of neuronal and oligodendroglial cytoplasmic inclusions in the pontine nuclei in multiple system atrophy. Acta Neuropathol. 96 (5): 439-44.
  • Lee HJ, et al. (2001) Membrane-bound alpha-Synuclein Has a High Aggregation Propensity and the Ability to Seed the Aggregation of the Cytosolic Form. The Journal of Biological Chemistry. 277: 671-8.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-alpha-Synuclein Antibody Rabbit Polyclonal IHC-P 106209-T08
    Anti-alpha-Synuclein Antibody Rabbit Polyclonal WB,ELISA,IP 12093-RP02
    Anti-alpha-Synuclein Antibody Rabbit Polyclonal ELISA 12093-RP01
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