Anti-SNAP-25 Antibody

Cat: 11585-RP02
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Anti-SNAP-25 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-SNAP-25 Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human SNAP-25
Immunogen
Recombinant Human SNAP25 protein (Catalog#11585-H07E)
Preparation
Produced in rabbits immunized with purified, recombinant Human SNAP25 (rh SNAP25; Catalog#11585-H07E; P60880-1; Met 1-Gly 206). SNAP25 specific IgG was purified by Human SNAP25 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-SNAP-25 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-SNAP-25 Antibody (Rabbit Polyclonal antibody) Images
Human SNAP25 Western blot (WB) 13418

Anti-SNAP25 rabbit polyclonal antibody at 1:500 dilution

Lane A: SH-SY5Y Whole Cell Lysate

Lane B: U87-MG Whole Cell Lysate

Lysates/proteins at 60 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:23 kDa

Observed band size:28 kDa

(We are unsure as to the identity of these extra bands.)

Anti-SNAP-25 Antibody Alternative Names
Anti-bA416N4.2 Antibody;Anti-dJ1068F16.2 Antibody;Anti-RIC-4 Antibody;Anti-RIC4 Antibody;Anti-SEC9 Antibody;Anti-SNAP Antibody;Anti-SNAP-25 Antibody
SNAP-25 Background Information

Synaptosomal-associated protein 25, also known as Super protein, Synaptosomal-associated 25 kDa protein, SNAP25 and SNAP, is a cytoplasm and cell membrane protein which belongs to the SNAP-25 family. SNAP25 / SUP contains 2 t-SNARE coiled-coil homology domains. SNAP25 / SUP is a membrane bound protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP25 / SUP protein is a component of the SNARE complex, which is proposed to account for the specificity of membrane fusion and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP25 / SUP is a Q-SNARE protein contributing two α-helices in the formation of the exocytotic fusion complex in neurons where it assembles with syntaxin-1 and synaptobrevin. SNAP25 / SUP is involved in the molecular regulation of neurotransmitter release. It may play an important role in the synaptic function of specific neuronal systems. SNAP25 / SUP associates with proteins involved in vesicle docking and membrane fusion. SNAP25 / SUP regulates plasma membrane recycling through its interaction with CENPF. SNAP25 / SUP inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP25 / SUP decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.

Full Name
synaptosomal-associated protein, 25kDa
References
  • Hodel A ,et al.,1998, Int. J. Biochem. Cell Biol. 30 (10): 1069-73.
  • Sudhof TC, et al.,2002, Nat Rev Neurosci 3 (8): 641-653.
  • Chapman ER et al.,2002, Nat. Rev. Mol. Cell Biol. 3(7): 498-508.
  • Chen X., et al., 2002, Neuron 33:397-409.
  • Huang Q., et al., 2008, FEBS Lett. 582:1431-1436.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-SNAP-25 Antibody Rabbit Polyclonal ELISA 11585-RP01
    Anti-SNAP-25 Antibody Rabbit Monoclonal ELISA 11585-R152
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