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SNAP25 Antibody ( Antigen Affinity Purified ) Datasheet
|Order or Inquire for SNAP25 Antibody product||Quality antibodies||Antibody production services|
|Detection limit is 0.5 ng/lane in WB|
|Detection limit is 0.00245 ng/well in ELISA|
SNAP25 Antibody Product Information
Recombinant Human SNAP25 protein (Catalog#11585-H07E)
|Antibody Type :||Rabbit Polyclonal Antibody ( Antibody Purification Platform )|
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS with 5% trehalose|
Produced in rabbits immunized with purified, recombinant Human SNAP25 (rh SNAP25; Catalog#11585-H07E; P60880-1; Met 1-Gly 206). SNAP25 specific IgG was purified by Human SNAP25 affinity chromatography.
SNAP25 Antibody Usage Guide
|Western blot :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SNAP25 in WB. Using a DAB detection system, the detection limit for Human SNAP25 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.|
|Direct ELISA :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SNAP25. The detection limit for Human SNAP25 is approximately 0.00245 ng/well.|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
SNAP25 Antibody Related Products & Topics
SNAP25 Antibody Background
Synaptosomal-associated protein 25, also known as Super protein, Synaptosomal-associated 25 kDa protein, SNAP25 and SNAP, is a cytoplasm and cell membrane protein which belongs to theSNAP-25 family. SNAP25 / SUP contains 2t-SNARE coiled-coil homology domains. SNAP25 / SUP is a membrane bound protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP25 / SUP protein is a component of the SNARE complex, which is proposed to account for the specificity of membrane fusion and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP25 / SUP is a Q-SNARE protein contributing two α-helices in the formation of the exocytotic fusion complex in neurons where it assembles with syntaxin-1 and synaptobrevin. SNAP25 / SUP is involved in the molecular regulation of neurotransmitter release. It may play an important role in the synaptic function of specific neuronal systems. SNAP25 / SUP associates with proteins involved in vesicle docking and membrane fusion. SNAP25 / SUP regulates plasma membrane recycling through its interaction with CENPF. SNAP25 / SUP inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP25 / SUP decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.
- Hodel A,et al.,1998, Int. J. Biochem. Cell Biol.30 (10): 1069-73.
- Sudhof TC, et al.,2002, Nat Rev Neurosci3 (8): 641-653.
- Chapman ER et al.,2002, Nat. Rev. Mol. Cell Biol.3 (7): 498-508.
- Chen X., et al., 2002, Neuron 33: 397-409.
- Huang Q., et al., 2008, FEBS Lett. 582: 1431-1436.
SNAP25 related areas, pathways, and other information