Anti-SNAP-25 Antibody (Rabbit Polyclonal antibody) General Information
Reacts with: Human
Recombinant Human SNAP25 protein (Catalog#11585-H07E)
Produced in rabbits immunized with purified, recombinant Human SNAP25 (rh SNAP25; Catalog#11585-H07E; P60880-1; Met 1-Gly 206). Total IgG was purified by Protein A affinity chromatography.
Polyclonal Rabbit IgG
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-SNAP-25 Antibody (Rabbit Polyclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-SNAP-25 Antibody Alternative Names
Anti-bA416N4.2 Antibody;Anti-dJ1068F16.2 Antibody;Anti-RIC-4 Antibody;Anti-RIC4 Antibody;Anti-SEC9 Antibody;Anti-SNAP Antibody;Anti-SNAP-25 Antibody
SNAP-25 Background Information
Synaptosomal-associated protein 25, also known as Super protein, Synaptosomal-associated 25 kDa protein, SNAP25 and SNAP, is a cytoplasm and cell membrane protein which belongs to the SNAP-25 family. SNAP25 / SUP contains 2 t-SNARE coiled-coil homology domains. SNAP25 / SUP is a membrane bound protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP25 / SUP protein is a component of the SNARE complex, which is proposed to account for the specificity of membrane fusion and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP25 / SUP is a Q-SNARE protein contributing two α-helices in the formation of the exocytotic fusion complex in neurons where it assembles with syntaxin-1 and synaptobrevin. SNAP25 / SUP is involved in the molecular regulation of neurotransmitter release. It may play an important role in the synaptic function of specific neuronal systems. SNAP25 / SUP associates with proteins involved in vesicle docking and membrane fusion. SNAP25 / SUP regulates plasma membrane recycling through its interaction with CENPF. SNAP25 / SUP inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP25 / SUP decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.
synaptosomal-associated protein, 25kDa
Hodel A ,et al.,1998, Int. J. Biochem. Cell Biol. 30 (10): 1069-73. Sudhof TC, et al.,2002, Nat Rev Neurosci 3 (8): 641-653. Chapman ER et al.,2002, Nat. Rev. Mol. Cell Biol. 3(7): 498-508. Chen X., et al., 2002, Neuron 33:397-409. Huang Q., et al., 2008, FEBS Lett. 582:1431-1436.