Mouse SMPD1 / ASM Protein (His Tag)

Sphingomyelin phosphodiesterase 1, acid lysosomal Protein Datasheet

SMPD1 / ASM Protein Product Information

• Synonym: A-SMase, ASM, Zn-SMase, aSMase
• Protein Construction: A DNA sequence encoding the mouse SMPD1 (Q04519) (Met 1-Leu 626) was expressed，with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Baculovirus

SMPD1 / ASM Protein QC Testing

• Purity: >85 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Leu 45
• Molecular Mass: The secreted recombinant mouse SMPD1 consists of 592 amino acids and has a calculated molecular mass of 66.3 kDa. It migrates as an approximately 63KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM Nacl, 10%glycerol, pH8.0, 0.1%Tween20
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: SMPD1 / ASM protein

SMPD1 / ASM Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SMPD1 / ASM Protein Description

Sphingomyelin phosphodiesterase 1 (SMPD1) , also known as ASM ( Acid sphingomyelinase ), is a member of the acid sphingomyelinase family of enzymes. Three isoforms have been identified, isoform 1 is 631 amino acids (aa) in length as the pro form, while Isoform 2 and isoform 3 have lost catalytic activity. The active SMPD1 isoform 1 contains one saposin B-type domain that likely interacts with sphingomyelin, and a catalytic region. Human SMPD1 is 86% aa identical to mouse SMPD1. SMPD1 is a monomeric lysosomal enzyme that converts sphingomyelin (a plasma membrane lipid ) into ceramide through the removal of phosphorylcholine. This generates second messenger components that participate in signal transduction. Defects in SMPD1 are the cause of Niemann-Pick disease type A (NPA) and type B (NPB), also known as Niemann-Pick disease classical infantile form and Niemann-Pick disease visceral form. Niemann-Pick disease is a clinically and genetically heterogeneous recessive disorder. NPB has little if any neurologic involvement and patients may survive into adulthood.

References

1. Schuchman EH. et al., 1992, Genomics. 12 (2): 197-205.
2. Ferlinz K. et al., 1997, Eur J Biochem. 243 (1-2): 511-7.
3. Lansmann S. et al., 2003, Eur J Biochem. 270: 1076-88.
4. Jones I. et al., 2008, Mol Genet Metab. 95 (3): 152-62.
5. Rodríguez-Pascau L. et al., 2009, Hum Mutat. 30 (7): 1117-22.
6. Bartelsen O. et al., 1998, Journal of Biotechnology. 63: 29-40.