SMAC / Diablo Antibody

SMAC / Diablo Antibody Datasheet

SMAC / Diablo Antibody Product Information

• Immunogen: Recombinant Human SMAC / Diablo protein (Catalog#10339-H08E)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 006
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human SMAC / Diablo (rh SMAC / Diablo; Catalog#10339-H08E; NP_063940.1; Ala 56-Asp 239).

SMAC / Diablo Antibody Usage Guide

• Specificity: Human SMAC / Diablo
• Western blot: This antibody can be used at 0.02-0.1 μg/mL with the appropriate secondary reagents to detect Human Diablo in WB.

  A B C D Sample (whole cell lysate) Hela Jurkat A431 MCF7 Sample Volume (cells/lane) 2.5×105 2.5×105 5×105 5×105 Gel 13% SDS-PAGE reducing gel Recommended Concentration 0.02-0.1 μg/ml Secondary Antibody Dylight 800-labeled Antibody To Rabbit IgG (H+L), at 1:5000 dilution.

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human Diablo.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

SMAC / Diablo Antibody Background

Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Apoptosis is carried out by caspases which reside in cells as inactive zymogens, and the initiator caspase zymogens are activated by adaptor proteins such as FADD and Apaf-1 mediated either by death receptors or cytochrome c. DIABLO (direct IAP binding protein with low pI), also well known as SMAC (second mitochondria derived activator of caspase), is a mitochondrial homodimeric protein, and performs a critical function in apoptosis by eliminating the inhibitory effect of IAPs (inhibitor of apoptosis proteins) on caspases. It has been shown that DIABLO mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. DIABLO is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs.

References

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5. Srinivasula, S.M. et al., 2000, J. Biol. Chem. 275: 36152-36157.
6. Goncalves, R.B. et al., 2008, Biochemistry. 25: 3832-3841.