Anti-SMAC Antibody

Cat: 10339-R006
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Anti-SMAC Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-SMAC Antibody
Validated applications
WB,ELISA,FCM,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human SMAC
Immunogen
Recombinant Human SMAC / Diablo protein (Catalog#10339-H08E)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human SMAC / Diablo (rh SMAC / Diablo; Catalog#10339-H08E; NP_063940.1; Ala 56-Asp 239).
Source
Monoclonal Rabbit IgG Clone #006
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-SMAC Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:10000-1:50000  
ELISA 1:5000-1:10000  
ICC-IF 1:20-1:100  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-SMAC Antibody (Rabbit Monoclonal antibody) Images
Human SMAC/Diablo  Western blot (WB) 13191

Anti-Diablo rabbit monoclonal antibody at 1:10000 dilution

Lane A: Hela Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lane C: A431 Whole Cell Lysate

Lane D: MCF7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:22 kDa

Observed band size:20 kDa

Human SMAC/Diablo  Immunofluorescence(IF) 14032
Immunofluorescence staining of Human Diablo in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Diablo monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Human SMAC Flow Cytometry (FC) 19136
Flow cytometric analysis of Human Diablo expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human Diablo (Filled hisgram), then a FITC-conjugated second step antibody. To demonstrate specificity of staining, the binding by 10339-R006 was blocked by preincubation of the purified antibody with 20ug recombinant human Diablo (Cat. No. 10339-H08E) for 1 hour (Black solid line hisgram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Anti-SMAC Antibody Alternative Names
Anti-DFNA64 Antibody;Anti-SMAC Antibody
SMAC Background Information

Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Second Mitochondria-Derived Activator of Caspases (Smac) or Direct IAP Binding Protein with low isoelectric point, pI (Diablo) is a proapoptogenic mitochondrial protein that is released to the cytosol in response to diverse apoptotic stimuli, including commonly used chemotherapeutic drugs. The current knowlege about structure and function of Smac/Diablo during programmed cell death, both in mitochondrial and receptor pathways are presented. It has been shown that Diablo mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. Diablo is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs. In the cytosol, Smac/Diablo interacts and antagonizes inhibitors of apoptosis proteins (IAPs), thus allowing the activation of caspases and apoptosis. This activity has prompted the synthesis of peptidomimetics that could potentially be used in cancer therapy. The role of Smac/DIABLO in colorectal carcinogenesis is ill defined. Data continues to accumulate to suggest that decreased levels of Smac/DIABLO may be important in chemoradiation-resistance to apoptosis in advanced colon cancer.

Full Name
diablo, IAP-binding mitochondrial protein
References
  • Korga A, et al. (2006) Role of mitochondrial protein Smac/Diablo in regulation of apoptotic pathways Pol Merkur Lekarski. 20(119): 573-6.
  • Anguiano-Hernandez YM, et al. (2007) Smac/DIABLO and colon cancer. Anticancer Agents Med Chem. 7(4): 467-73.
  • Martinez-Ruiz G, et al. (2008) Role of Smac/DIABLO in cancer progression. J Exp Clin Cancer Res. 27: 48.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-SMAC Antibody Mouse Monoclonal ICC/IF,IF 10339-MM12
    Anti-SMAC Antibody (FITC) Rabbit Monoclonal FCM 10339-R008-F
    Anti-SMAC Antibody Rabbit Polyclonal WB,ELISA,IP 10339-RP02
    Anti-SMAC Antibody Mouse Monoclonal WB,ELISA 10339-MM01
    Anti-SMAC Antibody Rabbit Monoclonal IP 10339-R001
    Anti-SMAC Antibody Rabbit Polyclonal WB,ELISA 10339-RP01
    Anti-SMAC Antibody Rabbit Monoclonal ELISA 10339-R002
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