- EGFR Signaling Pathway
- TGF-beta Signaling
- Canonical Wnt Signaling
- non-Canonical Wnt Signaling
- Notch Signaling
- p53 Pathway
- NF-kB Pathway
- Cytokine Signaling
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SMAC / Diablo Antibody Datasheet
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SMAC / Diablo Antibody Product Information
Recombinant Human SMAC / Diablo protein (Catalog#10339-H08E)
|Antibody Type :||Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )|
Clone ID :
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.|
This antibody was obtained from a rabbit immunized with purified, recombinant Human SMAC / Diablo (rh SMAC / Diablo; Catalog#10339-H08E; NP_063940.1; Ala 56-Asp 239).
SMAC / Diablo Antibody Usage Guide
Human SMAC / Diablo
|Western blot :||
This antibody can be used at 0.02-0.1 μg/mL with the appropriate secondary reagents to detect Human Diablo in WB.
|Direct ELISA :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human Diablo.|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
SMAC / Diablo Antibody Related Products & Topics
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|SMAC/Diablo||Human||SMAC/Diablo Protein, Recombinant||10339-H08E|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|WB, ELISA||SMAC/Diablo Antibody, Mouse MAb||10339-MM01|
|WB, ELISA||SMAC/Diablo Antibody, Rabbit PAb||10339-RP01|
|WB, ELISA||SMAC/Diablo Antibody, Rabbit PAb (Antigen Affinity Purified)||10339-RP02|
|WB, ELISA||SMAC / Diablo Antibody||10339-R006|
SMAC / Diablo Antibody Background
Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Apoptosis is carried out by caspases which reside in cells as inactive zymogens, and the initiator caspase zymogens are activated by adaptor proteins such as FADD and Apaf-1 mediated either by death receptors or cytochrome c. DIABLO (direct IAP binding protein with low pI), also well known as SMAC (second mitochondria derived activator of caspase), is a mitochondrial homodimeric protein, and performs a critical function in apoptosis by eliminating the inhibitory effect of IAPs (inhibitor of apoptosis proteins) on caspases. It has been shown that DIABLO mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. DIABLO is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs.
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- Du,C. et al., 2000, Cell. 102: 43-53.
- Adrain, C. et al., 2002, EMBO. J. 20: 6627-6636.
- Srinivasula, S.M. et al., 2000, J. Biol. Chem. 275: 36152-36157.
- Goncalves, R.B. et al., 2008, Biochemistry. 25: 3832-3841.