Rat SLC4A1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80390-G	Cloning vector ($295) pCMV / hygro ($545) pCMV / hygro - HA ($545) pCMV / hygro - His ($545) pCMV / hygro - Myc ($545) pCMV / hygro - FLAG ($545)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

SLC4A1 cDNA Clone Datasheet

SLC4A1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_012651.2
cDNA Size:	2787
Gene Synonym:	MGC93554; Slc4a1
Source:	Rat

SLC4A1 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus solute carrier family 4 (anion exchanger), member 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutations: 193 C/T resulting in the amino acid His substitution by Tyr and 2409 C/T not causing the amino acid variation.

SLC4A1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SLC4A1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SLC4A1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGGACA TGCAGGACCA CGAGAAAGTG CTGGAGATCC CAGACCGTGA CAGTGAAGAG
61       GAACTGGAAC ATGTAATAGA ACAGATAGCA TACAGAGACC TAGACATCCC TGTGACAGAA
121      ATGCAGGAGT CAGAAGCTCT TCCCACAGAG CAAACAGCCA CAGACTACAT CCCCACCAGT
181      ACCTCCACAT CGTACCCAAG CTCCAGTCAG GTCTATGTAG AGCTGCAGGA ACTGATGATG
241      GACCAGAGGA ACCAGGAACT ACAGTGGGTG GAGGCGGCAC ACTGGATAGG GCTGGAGGAA
301      AACCTTCGAG AGGATGGTGT GTGGGGTCGT CCACATCTCT CTTACCTCAC CTTCTGGAGC
361      CTTCTAGAAC TGCAGAAGGT CTTCTCAAAA GGCACCTTCC TTCTGGATCT GGCAGAGACA
421      TCCCTGGCTG GGGTGGCCAA TAAACTTCTA GACAGTTTCA TCTATGAGGA TCAAATTCGG
481      CCTCAAGACC GAGATGAGCT GCTCCGGGCT CTGCTACTCA AACGCAGCCA CGCTGAGGAC
541      CTAAAGGATC TGGAGGGAGT GAAGCCTGCC GTCCTGACCC GCTCTGGGGC CCCTTCTGAA
601      CCTCTTCTTC CTCACCAGCC ATCGCTGGAG ACCAAGCTGT ACTGTGCACA GGCAGAGGGG
661      GGCTCAGAAG AACCCTCGCC ATCTGGGATT CTGAAGATCC CCCCCAATTC AGAAACCACA
721      TTGGTGCTAG TGGGCCGGGC TAGTTTCCTG GTGAAGCCTG TGCTGGGCTT CGTGAGACTG
781      AAGGAAGCTG TGCCGCTGGA GGACCTGGTG TTGCCGGAGC CTGTGAGCTT CCTTCTAGTT
841      CTTCTCGGAC CTGAGGCTCC GCACATCGAC TACACCCAGC TGGGCAGGGC TGCAGCAACT
901      CTCATGACAG AAAGAGTGTT CCGCGTTACC GCCTCCCTGG CGCAGAGCCG AGGGGAGCTG
961      CTGAGCTCCC TGGATAGCTT TCTGGACTGT AGCCTGGTGC TGCCGCCCAC AGAGGCCCCT
1021     TCAGAGAAAG CTCTGCTCAA CCTGGTGCCC GTGCAGAAGG AGTTGCTTAG GAAGCGGTAC
1081     TTGCCCAGAC CTGCCAAGCC AGACCCCAAC TTGTACGAGG CATTAGCAGA CGGGGGAAAG
1141     GAGGGCCCCG GTGACGAAGA CGACCCTCTA AGGCGCACCG GCCGGATCTT TGGAGGCCTG
1201     ATCCGTGATA TCCGGCGCCG GTATCCTTAC TACCTGAGTG ACATCACCGA TGCGCTCAGT
1261     CCCCAGGTGC TGGCTGCTGT CATCTTCATC TACTTTGCTG CCTTGTCACC TGCGGTCACC
1321     TTCGGCGGTC TCCTGGGAGA AAAGACCCGG AACCTGATGG GGGTGTCAGA GCTGCTCATC
1381     TCCACAGCAG TGCAAGGAAT TCTCTTTGCT CTCCTGGGGG CACAGCCCCT GCTTGTGCTC
1441     GGCTTCTCAG GACCCCTGCT GGTGTTTGAA GAAGCCTTCT ACTCGTTCTG TGAAAGCAAT
1501     AACCTGGAGT ACATCGTGGG CCGCGCGTGG ATCGGCTTCT GGCTTATCCT GCTGGTGGTG
1561     TTGGTGGTGG CCTTTGAAGG CAGCTTCCTG GTCCAATACA TCTCCCGCTA CACTCAGGAG
1621     ATCTTCTCCT TCCTCATCTC CCTCATCTTC ATCTATGAGA CTTTCTCTAA GCTGATCAAG
1681     ATTTTCCAGG ACTACCCGCT ACAAGAGAGC TATGCCCCTG TTGTGATGAA GCCCAAACCT
1741     CAGGGCCCCG TGCCCAACAC AGCCCTCCTC TCCCTTGTGC TTATGGTTGG TACCTTCCTA
1801     CTTGCCATGA TGCTACGAAA GTTCAAGAAC AGCACCTACT TCCCCGGCAA GCTACGCAGA
1861     GTCATTGGGG ACTTTGGGGT CCCCATCTCC ATCCTGATAA TGGTCCTGGT GGATACCTTC
1921     ATCAAGAACA CCTACACGCA GAAACTCTCG GTGCCTGATG GCCTTAAAGT GTCCAACTCC
1981     TCCGCCCGGG GCTGGGTCAT CCATCCCCTG GGTCTGTATA ACCATTTCCC CAAGTGGATG
2041     ATGTTTGCTT CTGTTCTACC CGCTCTGCTT GTCTTCATCC TTATATTCCT TGAGTCTCAG
2101     ATCACCACGC TGATTGTCAG TAAACCTGAG CGGAAGATGA TCAAGGGCTC CGGCTTCCAC
2161     CTGGACTTGC TGCTGGTTGT TGGCATGGGC GGGGTGGCCG CCCTCTTTGG GATGCCTTGG
2221     CTCAGCGCCA CCACTGTGCG TTCTGTTACC CATGCCAACG CCCTCACAGT CATGGGGAAG
2281     GCCAGTGGTC CAGGGGCTGC AGCCCAGATC CAAGAGGTCA AGGAACAGCG GATCAGTGGG
2341     CTCCTGGTCT CTGTGCTCGT GGGACTGTCC ATTCTCATGG AACCCATCCT GTCCCGCATC
2401     CCCCTGGCTG TACTGTTTGG CATCTTCCTG TACATGGGGA TCACATCCCT CAGTGGCATC
2461     CAGCTCTTTG ACCGCATCTT GCTCCTGTTC AAGCCACCCA AGTACCACCC AGATGTGCCC
2521     TTTGTCAAGA GGGTGAAGAC CTGGCGTATG CACCTCTTCA CGGGCATCCA GATCATCTGC
2581     CTGGCTGTGC TGTGGGTGGT GAAGTCCACG CCAGCCTCGC TGGCCCTGCC CTTTGTCCTC
2641     ATCCTCACCG TGCCCCTGCG TCGTCTCCTC CTGCCGCTCA TCTTCAGAGA GCTAGAACTC
2701     CAGTGTCTGG ATGGTGATGA TGCCAAAGTG ACCTTTGACG AGGCGGAAGG GCTGGATGAG
2761     TATGACGAAG TGCCCATGCC TGTGTGA

Quality Control:cDNA sequencing results is available upon request.