Rat SLC44A1 Gene cDNA Clone / ORF Clone

SLC44A1 cDNA Clone Datasheet

SLC44A1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001033852.1
• cDNA Size: 1971
• Gene Synonym: Ctl1; Cdw92; Slc44a1
• Source: Rat

SLC44A1 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus solute carrier family 44, member 1 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 792 G/A not causing the amino acid variation.

SLC44A1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SLC44A1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SLC44A1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCTGCT GCAGCTCCGC CTCCGCCGCG CAGAGCTCCA AACGAGAATG GAAGCCGTTG
61       GAGGACCGTA GCTGCACAGA CATACCATGG CTGCTGCTCT TCGTTCTGTT CTGCATTGGG
121      ATGGGATTTA TTTGTGGCTT TTCAGTAGCA ACAGGTGCAG CAGCAAGACT AGTGTCAGGA
181      TACGACAGCT ATGGAAATAT CTGTGGGCAG AGAAATGCAA AACTGGAAGC AATAGCGAAC
241      AGTGGCCTGG ACCACACCCA CCGGAAGTAT GTGTTCTTTT TGGACCCATG CAATCTGGAC
301      TTGATAAACA GGAAGATCAA GTCTATGGCA CTGTGTGTAG CGGCATGTCC ACGACAGGAG
361      TTGAAAACCC TGAGTGACGT CCAGAAGTTT GCCGAGATCA ATGGTTCAGC ACTGTGTAGC
421      TACAACATAA AGCCTTCCGA GTACACTCTG ACTGCAAAAT CCTCTGCCTT TTGCCCCAAA
481      CTACCAGTAC CAGCCAGTGC ACCTATTCCA TTCTTCCATC GCTGTGCTCC TGTGAACATT
541      TCCTGCTATG CCAAGTTTGC AGAGGCCCTG ATCACCTTTG TCAGTGACAA TAGTGTCTTA
601      CACAGGCTGA TTAGTGGAGT AATGACCAGC AAAGAAATTA TATTGGGACT TTGCTTGTTA
661      TCACTAGTTC TGTCCATGAT TTTGATGGTG ATTATCCGGT ATATCTCAAG AGTGCTAGTG
721      TGGATCCTAA CGATTCTGGT CATCCTGGGT TCACTAGGTG GCACCGGTGT ACTATGGTGG
781      CTATATGCAA AACAGAGAAG GTCCCCCAAA GAAACAGTTA TTCCTGAACA GCTTCAGATC
841      GCGGAAGACA ATCTGCGGGC GCTCCTCATC TATGCCATTT CTGCTACGGT GTTCACTGTC
901      ATTCTCTTCC TGATCATGCT GGTTATGCGT AAACGCGTCG CTCTCACCAT CGCCTTGTTC
961      CACGTGGCTG GCAAGGTCTT CATACACCTG CCTCTGCTAG TCTTCCAGCC CTTCTGGACT
1021     TTTTTTGCTC TCGTCTTATT TTGGGCATAC TGGATAATGA CACTTCTCTT CCTTGGCACT
1081     ACTGGCAGTG CTGTTCAGAA TGAACAAGGT TTCGTGGAGT ACAAAATCTC TGGGCCTTTG
1141     CAGTACATGT GGTGGTACCA CGTGGTCGGC CTGATTTGGA TCAGTGAGTT CATTCTCGCC
1201     TGTCAGCAGA TGACTGTGGC AGGAGCTGTG GTAACATACT ACTTTACAAG GGATAAACGG
1261     AATTTGCCTT TCACACCTAT TCTGGCATCA GTGAACCGCC TTATTCGTTA CCACCTCGGT
1321     ACTGTGGCCA AAGGATCCTT CATTATCACC CTCGTCAAGA TCCCCAGGAT GATCCTTATG
1381     TATATCCACA GTCAGCTCAA AGGGAAGGAA AACGCTTGTG CACGATGCAT GCTGAAATCC
1441     TGCATTTGCT GCCTTTGGTG TCTTGAAAAG TGCCTGAGCT ACTTAAACCA GAATGCATAC
1501     ACAGCCACAG CCATCAATAG CACCAACTTC TGCACCTCAG CAAAGGATGC CTTCGTCATT
1561     CTAGTGGAGA ATGCTCTGCG AGTGGCTGCC ATCAACACAG TGGGGGACTT CATGTTATTC
1621     CTAGGCAAGG TGCTGATAGT CTGCAGCACG GGCCTGGCTG GGATTATGCT GCTCAACTAC
1681     CAGCAAGATT ACACAGTGTG GGTGCTGCCT CTCATCATCG TCTGCCTCTT TGCTTTCCTA
1741     GTCGCTCATT GCTTCCTGTC CATTTACGAA ATGGTAGTGG ATGTGTTGTT TTTGTGTTTT
1801     GCCATTGATA CAAAATACAA TGATGGGAGC CCAGGCCGAG AATTCTATAT GGACAAAGTC
1861     CTAATGGAGT TTGTGGAAAA CAGTAGGAAA GCAATGAAAG AAGCTGGTAA GGGAGGCGCC
1921     GCGGATGCCC GAGAGCTAAA GCCGATGGCT TCGGGAGCAA GTTCTGCTTG A

 

Quality Control:cDNA sequencing results is available upon request.