Rat SLC3A2 Gene cDNA Clone / ORF Clone

SLC3A2 cDNA Clone Datasheet

SLC3A2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_019283.1
• cDNA Size: 1584
• Gene Synonym: Mdu1; Slc3a2
• Source: Rat

SLC3A2 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 714 G/T not causing the amino acid variation.

SLC3A2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SLC3A2 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SLC3A2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGCCAGG ACACCGAAGT GGACATGAAA GATGTGGAGC TGAACGAGCT GGAACCGGAG
61       AAGCAGCCTA TGAATGCAGC GGACGGGGCG GCAGCCGGGG AGAAGAACGG TCTGGTGAAG
121      ATTAAGGTGG CCGAAGACGA GGCGGAAGCC GGGGTCAAGT TCACAGGCTT ATCCAAGGAG
181      GAGCTATTGA AGGTAGCTGG CAGCCCGGGC TGGGTGCGCA CCCGCTGGGC GCTGCTGCTG
241      CTCTTCTGGC TCGGTTGGCT GGGTATGCTG GCGGGCGCCG TGGTTATCAT CGTTCGGGCG
301      CCACGCTGCC GTGAGCTGCC GGTACAGAGA TGGTGGCACA AGGGCGCCCT CTACCGCATC
361      GGCGACCTTC AGGCCTTCGT AGGCCCGGAA GCGAGAGGCA TAGCTGGTCT GAAGAACCAT
421      CTGGAGTACT TGAGCACCCT GAAGGTGAAG GGCCTAGTTT TGGGCCCAAT TCACAAGAAC
481      CAGAAGGATG AAGTCAATGA AACCGACTTG AAACAGATTG ATCCCGATTT AGGCTCCCAG
541      GAAGATTTTA AAGACCTTCT ACAAAGTGCC AAGAAAAAGA GCATTCACAT CATTTTGGAC
601      CTCACTCCCA ACTATAAGGG CCAGAATGCA TGGTTCCTCC CTCCTCAGGC TGACATTGTA
661      GCCACCAAAA TGAAGGAGGC TCTGAGTTCT TGGTTGCAGG ACGGTGTGGA TGGTTTCCAA
721      GTTCGGGATG TGGGAAAGCT GGCGAATGCA TCCTTGTACT TGGCTGAGTG GCAGAATATC
781      ACCAAGAACT TCAGTGAGGA CAGGCTTTTG ATTGCAGGGA CCGCGTCCTC TGACCTGCAA
841      CAAATTGTCA ACATACTTGA ATCCACCAGC GATCTGCTGC TGACCAGCTC ATACCTGTCA
901      CAGCCCGTTT TCACTGGGGA GCATGCAGAA CTCCTAGTGA TTAAGTATTT GAATGCCACT
961      GGCAGCCGCT GGTGCAGCTG GAGTGTGTCG CAGGCAGGAC TCCTGACATC CTTTATACCG
1021     GCTCAGTTTC TCCGACTCTA CCAGCTGCTG CTCTTCACTC TGCCAGGAAC TCCTGTTTTC
1081     AGCTATGGGG ATGAGCTTGG CCTTCAGGCA GTTGCCCTTC CTGGACAGCC TATGGAGGCT
1141     CCATTCATGC TGTGGAATGA GTCTAGCAAC TCCCAAACCT CAAGTCCTGT AAGCCTCAAC
1201     ATGACAGTGA AGGGCCAAAA TGAAGACCCC GGCTCCCTCC TCACCCAGTT CCGGCGACTG
1261     AGTGACCTCC GTGGTAAGGA GCGCTCTCTG TTACACGGTG ACTTTGATGC ACTGTCTTCC
1321     TCATCTGGCC TCTTCTCCTA CGTCCGCCAC TGGGACCAGA ATGAGCGTTA CCTGGTGGTG
1381     CTCAACTTCC AGGATGTGGG CCTGTCAGCC AGGGTAGGAG CCTCCAACCT CCCTGCTGGC
1441     ATAAGCCTGC CAGCCAGTGC TAACCTTTTG CTTAGTACTG ACAGCACCCG GCTAAGCCGT
1501     GAGGAGGGCA CCTCCCTGAG CCTGGAAAAC CTGAGCCTGA ATCCTTATGA GGGCTTGTTG
1561     TTACAGTTCC CTTTTGTGGC CTGA

 

Quality Control:cDNA sequencing results is available upon request.