Human SLC27A5 Gene cDNA Clone / ORF Clone

Human SLC27A5 cDNA Clone Datasheet

SLC27A5 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_012254.2
• cDNA Size: 2073
• Gene Synonym: ACSB; FATP5; ACSVL6; FACVL3; VLACSR; VLCSH2; VLCS-H2; FLJ22987; SLC27A5
• Source: Human

SLC27A5 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens solute carrier family 27 (fatty acid transporter), member 5 DNA.
• Vector: pGEM-T Vector

• Sequence Description: Same as Gene Bank Ref. ID sequence with the nucleotide 436-687 deletion mutation and the point mutation 149 T/C and 157 C/T, resulting in the amino acid Met substitution by Thr,Arg substitution by Trp.

SLC27A5 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SLC27A5 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SLC27A5 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGTGTCA GGCAACAGTT GGCCTTGCTG CTGCTGCTGC TGCTCCTGCT CTGGGGCCTG
61       GGGCAGCCAG TGTGGCCAGT CGCTGTGGCC TTGACCCTGC GCTGGCTCCT GGGGGATCCC
121      ACATGTTGCG TGCTACTTGG GCTGGCCACG TTAGCATGGC CCTGGCTCGG CCCCTGGGTG
181      CCCCATGGGC TGAGCCTGGC AGCTGCGGCC CTGGCACTAA CCCTCCTGCC AGCACGGCTG
241      CCCCCAGGAC TACGCTGGCT GCCGGCTGAT GTGATCTTCT TGGCCAAGAT CCTCCACCTG
301      GGCCTGAAGA TCAGGGGATG CTTGAGCCGG CAGCCGCCTG ACACCTTTGT AGATGCCTTC
361      GAGCGGCGAG CACGAGCGCA GCCTGGCAGG GCACTCTTGG TGTGGACGGG GCCTGGGGCC
421      GGCTCAGTCA CCTTTGACCT CCGGGAGAGC CTGGAGGAGA TCCTTCCCAA GCTGCAGGCT
481      GAGAACATCC GCTGCTTCTA CCTCAGCCAT ACCTCCCCTA CACCAGGGGT GGGGGCTCTG
541      GGGGCTGCCC TGGATGCAGC GCCCTCCCAC CCAGTGCCTG CTGACCTGCG TGCTGGGATC
601      ACATGGAGAA GCCCTGCCCT CTTCATCTAT ACCTCGGGGA CCACTGGCCT CCCGAAGCCA
661      GCCATCCTCA CGCATGAGCG GGTACTGCAG ATGAGCAAGA TGCTGTCCTT ATCTGGGGCC
721      ACAGCTGATG ATGTGGTTTA CACGGTCCTG CCTCTGTACC ACGTGATGGG ACTTGTCGTT
781      GGGATCCTCG GCTGCTTAGA TCTCGGAGCC ACCTGTGTTC TGGCCCCCAA GTTCTCTACT
841      TCCTGCTTCT GGGATGACTG TCGGCAGCAT GGCGTGACAG TGATCCTGTA TGTGGGCGAG
901      CTCCTGCGGT ACTTGTGTAA CATTCCCCAG CAACCAGAGG ACCGGACACA TACAGTCCGC
961      CTGGCAATGG GCAATGGACT ACGGGCTGAT GTGTGGGAGA CCTTCCAGCA GCGCTTCGGT
1021     CCTATTCGGA TCTGGGAAGT CTACGGCTCC ACAGAAGGCA ACATGGGCTT AGTCAACTAT
1081     GTGGGGCGCT GCGGGGCCCT GGGCAAGATG AGCTGCCTCC TCCGAATGCT GTCCCCCTTT
1141     GAGCTGGTGC AGTTCGACAT GGAGGCGGCG GAGCCTGTGA GGGACAATCA GGGCTTCTGC
1201     ATCCCTGTAG GGCTAGGGGA GCCGGGGCTG CTGCTGACCA AGGTGGTAAG CCAGCAACCC
1261     TTCGTGGGCT ACCGCGGCCC CCGAGAGCTG TCGGAACGGA AGCTGGTGCG CAACGTGCGG
1321     CAATCGGGCG ACGTTTACTA CAACACCGGG GACGTACTGG CCATGGACCG CGAAGGCTTC
1381     CTCTACTTCC GCGACCGCCT CGGGGACACC TTCCGATGGA AGGGCGAGAA CGTGTCCACG
1441     CACGAGGTGG AGGGCGTGTT GTCGCAGGTG GACTTCTTGC AACAGGTTAA CGTGTATGGC
1501     GTGTGCGTGC CAGGTTGTGA GGGTAAGGTG GGCATGGCTG CTGTGCAGCT AGCCCCCGGC
1561     CAGACTTTCG ACGGGGAGAA GTTGTACCAG CACGTTCGCG CTTGGCTCCC TGCCTACGCT
1621     ACCCCCCATT TCATCCGCAT CCAGGACGCC ATGGAGGTCA CCAGCACGTT CAAACTGATG
1681     AAGACCCGGT TGGTGCGTGA GGGCTTCAAT GTGGGGATCG TGGTTGACCC TCTGTTTGTA
1741     CTGGACAACC GGGCCCAGTC CTTCCGGCCC CTGACGGCAG AAATGTACCA GGCTGTGTGT
1801     GAGGGAACCT GGAGGCTCTG A

 

Quality Control:cDNA sequencing results is available upon request.