Rat SLAMF1 Gene cDNA Clone / ORF Clone

SLAMF1 cDNA Clone Datasheet

SLAMF1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001109078.1
• cDNA Size: 1038
• Gene Synonym: RGD1560634; Slamf1
• Source: Rat

SLAMF1 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus signaling lymphocytic activation molecule family member 1 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 535 G/A resulting in the amino acid Gly substitution by Ser.

SLAMF1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SLAMF1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SLAMF1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGATCCCA ACCGATCCCT TTCCTTGAGA ATACTTCTGT TTCTCTCCCT GGCTGTTGAG
61       TTGAGCTACG GAACAGGTAC AGGTATGATG GACTGCCCGG TGATTCTCCA GGAGCTGGGA
121      CGTGACACAT GGCTGGCCCT GACAAATGAA CATCAGATTA ATAAGAGCGC GAACAAAAGC
181      ATCCGCATCC TTGTCACGAT GGCAAGGTCT CCAGGAAGCA AAACCAACAA GAAAATAGTG
241      TCTTTTGATC TCTCGAAAGG GGGCTACCCA GATCACCTGG AGGATGGCTA CCAGTTTCAA
301      TCCAAAAACC TAAGCCTGAA GATCCTAGGG AACAGGAGGG AGAGTGAAGG CTTGTACTTT
361      GTGACCGTGG AAGAGAACGT GTCAGTCCAT CAATCCTGCA CGCAACTGAA GCTTTATGAA
421      CAGGTCTCCA CTCCAGAGAT TAAAGTGCTA AACAAAACCC AGGAGGAGAA TGGGACCTGC
481      CACTTGCTGC TAGCCTGCAC GGTGAAGAGA GGGGATAATG TGTCTTACAG CTGGAGTGAT
541      GAGGCAGGTA CCCGTCTGCC AAGCCAAGCC AACCGCTCCC ACCTCCTGCA CCTCATCCTT
601      AGCAACCAGC ATCAGGACAG CATCTACAAC TGCACAGCGA GCAATCCTGT CAGCAGTCGC
661      TCTCGGACCT TCAACCTATG GCAGCAATGC AGGCCAGAAT CCTACTCAGA ATCGAGTCTG
721      TGGCTTCCGT ATATTGCTGC ACCATTAGGG GTCATCATAT TCGTCATCCT GGTTTCCATA
781      GCAACAGTAT TGGTGAAAAG ATACTGGGCT CCCAGTGGTA AACCAAATCA CTGCCAGCCA
841      CCTGTAGAAG AAAAAAGCCT TACTATCTAT GCCCAAGTAC AGAAATCAGG GCCTCTAGAG
901      AAGGAACTTC ATGATGCCCT GACAGATCAG GACCCCTGCA CGACCATTTA TGTGGCTGCC
961      ACAGAGCCTG TCCCAGAATC TGTCCAGGAA CCAAACCCCA CCACAGTTTA TGCCAGTGTG
1021     ACACTGCCAG AGAGCTGA

 

Quality Control:cDNA sequencing results is available upon request.