Anti-SIRT1 Antibody

Cat: 11748-R012
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Anti-SIRT1 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-SIRT1 Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Human
Specificity
Human SIRT1
Immunogen
Recombinant Human SIRT1 protein (Catalog#11748-H07E)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human SIRT1 (rh SIRT1; Catalog#11748-H07E; NP_036370.2; Met 193-Ser 747).
Source
Monoclonal Rabbit IgG Clone #012
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-SIRT1 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-SIRT1 Antibody Alternative Names
Anti-SIR2L1 Antibody
SIRT1 Background Information

SIRT1 belongs to the sirtuin family. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. SIRT1 is included in class I of the sirtuin family. It is a NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. It deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. SIRT1 also deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. It is involved in HES1- and HEY2-mediated transcriptional repression. SIRT1 inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. It may serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. It also deacetylates 'Lys-16' of histone H4 (in vitro). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus.

Full Name
sirtuin 1
References
  • Sharma A, et al. (2012) Interactomic and pharmacological insights on human Sirt-1. Front Pharmacol. 3-40.
  • Sun C, et al. (2007) SIRT1 improves insulin sensitivity under insulin-resistant conditions by repressing PTP1B. Cell Metab. 6(4):307-19.
  • Rodgers JT, et al. (2005) Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1. Nature. 434(7029):113-8.
  • Nemoto S, et al. (2005) SIRT1 functionally interacts with the metabolic regulator and transcriptional coactivator PGC-1{alpha}. J Biol Chem. 280(16):16456-60.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-SIRT1 Antibody Rabbit Monoclonal ELISA 11748-R032
    Anti-SIRT1 Antibody Rabbit Polyclonal WB,ELISA,ICC/IF,IF,IP 11748-T54
    Anti-SIRT1 Antibody Rabbit Polyclonal WB,IP 100799-T36
    Anti-SIRT1 Antibody Rabbit Polyclonal WB,IP 11748-T36
    Anti-SIRT1 Antibody Rabbit Polyclonal ELISA 11748-RP01
    Anti-SIRT1 Antibody Mouse Monoclonal ELISA,IHC-P,ICC/IF,IF 11748-MM04
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