SIRT1 Antibody

SIRT1 Antibody Datasheet

SIRT1 Antibody Product Information

• Immunogen: Recombinant Human SIRT1 protein (Catalog#11748-H07E)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 102
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human SIRT1 (rh SIRT1; Catalog#11748-H07E; NP_036370.2; Met 193-Ser 747).

SIRT1 Antibody Usage Guide

• Specificity: Human SIRT1
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SIRT1. The detection limit for Human SIRT1 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

SIRT1 Antibody Background

NAD-dependent deacetylase sirtuin-1, also known as SIR2-like protein 1, SIRT1 and SIR2L1, is a nucleus protein which belongs to thesirtuin family. SIRT1 / SIR2L1 is widely expressed and contains onedeacetylase sirtuin-type domain. Mammalian SIRT1 is an NAD-dependent deacetylase with critical roles in the maintenance of homeostasis and cell survival. Elevated levels of SIRT1 protein are evident in cancer in which SIRT1 can function as a cancer-specific survival factor. SIRT1 / SIR2L1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. It deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. SIRT1 / SIR2L1 deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. It is involved in HES1- and HEY2-mediated transcriptional repression. It inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. SIRT1 / SIR2L1 may serve as a sensor of the cytosolic ratio of NAD+/NADH, which is essential in skeletal muscle cell differentiation. In case of HIV-1 infection, it interacts with and deacetylates the viral Tat protein.

References

1. Vaziri H. et al., 2001, Cell 107: 149-59.
2. Langley E. et al., 2002, EMBO J. 21: 2383-96.
3. Bitterman K.J. et al., 2002, J. Biol. Chem. 277: 45099-107.
4. Takata T. et al., 2003, Biochem. Biophys. Res. Commun. 301: 250-7.
5. Pagans S. et al., 2005, PLoS Biol. 3: 210-20.
6. Ford,J. et al., 2008, Cell Cycle. 7 (19): 3091-7.