SIRPG / SIRP gamma / CD172g Antibody

SIRPG / SIRP gamma / CD172g Antibody Datasheet

SIRPG / SIRP gamma / CD172g Antibody Product Information

• Immunogen: Recombinant Human SIRPG / SIRP gamma / CD172g protein (Catalog#11828-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 1H7C2
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SIRPG / SIRP gamma / CD172g (rh SIRPG / SIRP gamma / CD172g; Catalog#11828-H08H; NP_061026.2; Met 1-Ser 364). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

SIRPG / SIRP gamma / CD172g Antibody Usage Guide

• Specificity: Human SIRPG / SIRP gamma / CD172g
• No cross-reactivity in ELISA with

  Human cell lysate (293 cell line)

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human SIRPG in WB. Using a DAB detection system, the detection limit for Human SIRPG is approximately 1 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human SIRPG. The detection limit for Human SIRPG is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

SIRPG / SIRP gamma / CD172g Antibody Background

Signal-regulatory protein gamma, also known as SIRP-gamma, CD172 antigen-like family member B, Signal-regulatory protein beta-2, SIRP-beta-2, CD172g and SIRPG, is a single-pass type I membrane protein. SIRPG / CD172g is detected in liver, and at very low levels in brain, heart, lung, pancreas, kidney, placenta and skeletal muscle. It is expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells. SIRPG / CD172g contains twoIg-like C1-type (immunoglobulin-like) domains and oneIg-like V-type (immunoglobulin-like) domain. It is mainly present in the paracortical T-cell area of lymph nodes, with only sparse positive cells in the mantle and in the germinal center of B-cell follicles. In the thymus, SIRPG / CD172g is primarily expressed in the medulla on mature T lymphocytes that have undergone thymic selection. SIRPG / CD172g is probable immunoglobulin-like cell surface receptor. On binding with CD47, it mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation.

References

1. Ichigotani Y., et al., 2000, J. Hum. Genet. 45:378-82.
2. Van den Berg TK, et al., 2005, J Immunol. 175 (12): 7788-9.
3. Piccio L., et al., 2005, Blood. 105: 2421-7.
4. Hatherley D., et al., 2008, Mol. Cell. 31:266-77.