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Human SIRPB1 Gene cDNA Clone / ORF Clone

Catalog Vector (Price) Quantity In Stock Operation
HG13770-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

SIRPB1 cDNA Clone Datasheet

 

SIRPB1 cDNA Clone Product Information

Gene Bank Ref.ID: BC025286

cDNA Size:

546

Gene Synonym:

RP4-576H24.1; CD172b; DKFZp686A05192; FLJ26614; SIRP-BETA-1; SIRPB1

Source: Human

SIRPB1 cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens signal-regulatory protein beta 1 DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref.ID sequence.

SIRPB1 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SIRPB1 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
  3. Transfer the tube to incubation at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SIRPB1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCCGTGC CAGCCTCCTG GCCCCACCTT CCTAGTCCTT TCCTGCTGAT GACGCTACTG
61       CTGGGGAGAC TCACAGGAGT GGCAGGTGAG GACGAGCTAC AGGTGATTCA GCCTGAAAAG
121      TCCGTATCAG TTGCAGCTGG AGAGTCGGCC ACTCTGCGCT GTGCTATGAC GTCCCTGATC
181      CCTGTGGGGC CCATCATGTG GTTTAGAGGA GCTGGAGCAG GCCGGGAATT AATCTACAAT
241      CAGAAAGAAG GCCACTTCCC ACGGGTAACA ACTGTTTCAG AACTCACAAA GAGAAACAAC
301      CTGGACTTTT CCATCAGCAT CAGTAACATC ACCCCAGCAG ACGCCGGCAC CTACTACTGT
361      GTGAAGTTCC GGAAAGGGAG CCCTGACGAC GTGGAGTTTA AGTCTGGAGC AGGCACTGAG
421      CTGTCTGTGC GCGAACCAGC GCTGGCTCCT ACTGCTCCAC TCCTCGTAGC TCTCCTCCTG
481      GGCCCCAAGC TGCTACTGGT GGTTGGTGTC TCTGCCATCT ACATCTGCTG GAAACAGAAG
541      GCCTGA

 

Quality Control:cDNA sequencing results is available upon request.