Rat SIRPA Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80270-G	Cloning vector ($195) pCMV / hygro ($395) pCMV / hygro - HA ($395) pCMV / hygro - His ($395) pCMV / hygro - Myc ($395) pCMV / hygro - FLAG ($395)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

SIRPA cDNA Clone Datasheet

SIRPA cDNA Clone Product Information

Gene Bank Ref.ID:	NM_013016.2
cDNA Size:	1530
Gene Synonym:	Bit; Ptpns1; SHPS-1; Sirpa
Source:	Rat

SIRPA cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus signal-regulatory protein alpha DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutations: 73 A/T, 565 A/G, 485 G/C resulting in the amino acid Ile substitution by Phe, Asn substitution by Asp, Gly substitution by Ala and 54 T/G not causing the amino acid variation.

SIRPA cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SIRPA cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SIRPA cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGCCCG CCGGCCCGGC CCCTGGCCGC CTAGGGCCGC TGCTGTTCTG CCTGCTGCTC
61       TCCGCCTCCT GTTTCTGTGC AGGAGCCAGC GGGAAAGAAC TGAAGGTGAC TCAGGCTGAC
121      AAATCAGTGT CTGTTGCTGC TGGAGATTCG GCCACTCTGA ACTGCACTGT GTCCTCCCTG
181      ACGCCTGTGG GACCCATTAA GTGGTTCAAA GGAGAAGGGC AAAATCGGAG CCCGATCTAC
241      AGTTTCATAG GAGGAGAACA CTTTCCTCGA ATTACAAATG TTTCAGATGC TACTAAGAGA
301      AACAATATGG ACTTTAGCAT CTGTATCAGT AATGTCACCC CAGAAGATGC TGGCACCTAC
361      TACTGTGTGA AGTTCCAGAA AGGAATAGTA GAGCCTGACA CAGAAATTAA ATCTGGAGGG
421      GGAACAACGC TCTATGTACT CGCCAAACCT TCTTCACCGG AAGTATCGGG CCCAGACTCC
481      AGGGCCTCTC CTGGACAGAC AGTGAACTTC ACCTGCAAGT CTTACGGCTT CTCTCCCCGG
541      AATATCACCC TGAAGTGGCT CAAAGATGGG AAAGAACTCT CCCATTTGGA GACCACCATC
601      TCCAGTAAAA GCAATGTCTC CTACAACATC TCCAGCACAG TCAGCGTGAA ACTAAGCCCC
661      GAGGACATTC ATTCTCGGGT CATCTGCGAG GTAGCCCACG TCACCTTGGA AGGACGCCCG
721      CTTAATGGGA CCGCTAACTT TTCTAACATC ATCCGAGTTT CACCCACCTT GAAGATCACC
781      CAACAGCCCC TGACGCCCGC GAGCCAGGTG AACCTCACCT GCCAGGTGCA GAAGTTCTAC
841      CCCAAGGCTC TCCAGCTGAA CTGGCTGGAG AATGGAAACT TATCACGGAC GGACAAGCCC
901      GAGCATTTCA CAGACAACAG GGATGGGACC TATAATTACA CAAGCCTGTT CCTGGTGAAC
961      TCATCTGCTC ACAGAGAGGA TGTGGTATTC ACGTGCCAGG TGGAGCATGA CAGTCAGCCA
1021     GCGATCACCG AAAACCATAC CGTGCGGGCA TTTGCCCACT CGAGTAGTGG AGGCAGCATG
1081     GAAACCATCC CTGATAATAA TGCTTACTAC AACTGGAACG TCTTCATCGG TGTGGGTGTG
1141     GCGTGTGCTT TGCTAGTAGT CCTGCTGATG GCTGCCCTCT ACCTCCTCCG AATCAAACAG
1201     AAGAAAGCCA AGGGCTCAAC TTCTTCCACA CGGTTGCACG AGCCCGAGAA GAATGCCAGG
1261     GAAATAACCC AGATCCAGGA CACAAATGAC ATCAACGACA TCACATACGC AGACCTGAAT
1321     CTGCCCAAAG AGAAGAAGCC CGCCCCCCGG GTCCCCGAGC CCAACAACCA CACAGAATAT
1381     GCAAGCATTG AGACAGGCAA ACTGCCTAGG CCAGAGGATA CCCTCACCTA TGCTGACCTG
1441     GACATGGTCC ACCTCAACCG GGCACAGCCA ACCCCCAAGC CTGAGCCATC CTTCTCAGAG
1501     TATGCCAGTG TCCAAGTCCA GAGGAAGTGA

Quality Control:cDNA sequencing results is available upon request.