Mouse SIRPA Gene cDNA Clone / ORF Clone

SIRPA cDNA Clone Datasheet

SIRPA cDNA Clone Product Information

• Gene Bank Ref.ID: NM_007547.2
• cDNA Size: 1530
• Gene Synonym: Bit; P84; SIRP; SHP-1; CD172a; Ptpns1
• Source: Mouse

SIRPA cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus signal-regulatory protein alpha DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 459 A/G not causing the amino acid variation.

SIRPA cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SIRPA cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SIRPA cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGCCCG CCGGCCCGGC CCCTGGCCGC CTAGGGCCGC TGCTGCTCTG CCTGCTGCTC
61       TCCGCGTCCT GTTTCTGTAC AGGAGCCACG GGGACAGAAG TGAAGGTGAC TCAGCCTGAG
121      AAATCAGTGT CTGTTGCTGC TGGGGATTCG ACCATTCTCA ACTGCACTGT GACCTCCTTG
181      TTGCCGGTGG GACCCATTAG GTGGTACAGA GGAGTAGGGC AAAGCCGGCT GTTGATCTAC
241      AGTTTCACAG GAGAACACTT TCCTCGAGTT AGAAATGTTT CAGATACTAC TAAGAGAAAC
301      AATATGGACT TTTCCATCCG TATCAGTAAT GTCACCCCAG AAGATGCCGG TACCTACTAC
361      TGTGTGAAGT TCCAGAGAGG ATCATCAGAG CCTGACACAG AAATACAATC TGGAGGGGGA
421      ACAGAGGTCT ATGTACTCGC CAAACCTTCT CCACCGGAGG TATCCGGCCC AGCAGACAGG
481      GGCATACCTG ACCAGAAAGT GAACTTCACC TGCAAGTCTC ATGGCTTCTC TCCCCGGAAT
541      ATCACCCTGA AGTGGTTCAA AGATGGGCAA GAACTCCACC CCTTGGAGAC CACCGTGAAC
601      CCTAGTGGAA AGAATGTCTC CTACAACATC TCCAGCACAG TCAGGGTGGT ACTAAACTCC
661      ATGGATGTTA ATTCTAAGGT CATCTGCGAG GTAGCCCACA TCACCTTGGA TAGAAGCCCT
721      CTTCGTGGGA TTGCTAACCT GTCTAACTTC ATCCGAGTTT CACCCACCGT GAAGGTCACC
781      CAACAGCCCC CGACATCAAT GAACCAGGTG AACCTCACCT GCCGGGCTGA GAGGTTCTAC
841      CCCGAGGATC TCCAGCTGAT CTGGCTGGAG AATGGAAACG TATCACGGAA TGACACGCCC
901      AAGAATCTCA CAAAGAACAC GGATGGGACC TATAATTACA CAAGCTTGTT CCTGGTGAAC
961      TCATCTGCTC ATAGAGAGGA CGTGGTGTTC ACGTGCCAGG TGAAGCACGA CCAACAGCCA
1021     GCGATCACCC GAAACCATAC CGTGCTGGGA CTTGCCCACT CGAGTGATCA AGGGAGCATG
1081     CAAACCTTCC CTGGTAATAA TGCTACCCAC AACTGGAATG TCTTCATCGG TGTGGGCGTG
1141     GCGTGTGCTT TGCTCGTAGT CCTGCTGATG GCTGCTCTCT ACCTCCTCCG GATCAAACAG
1201     AAGAAAGCCA AGGGGTCAAC ATCTTCCACA CGGTTGCACG AGCCCGAGAA GAATGCCAGG
1261     GAAATAACCC AGATCCAGGA CACAAATGAC ATCAACGACA TCACATACGC AGACCTGAAT
1321     CTGCCCAAAG AGAAGAAGCC CGCACCCCGG GCCCCTGAGC CTAACAACCA CACAGAATAT
1381     GCAAGCATTG AGACAGGCAA AGTGCCTAGG CCAGAGGATA CCCTCACCTA TGCTGACCTG
1441     GACATGGTCC ACCTCAACCG GGCACAGCCA GCCCCCAAGC CTGAGCCATC TTTCTCAGAG
1501     TATGCTAGTG TCCAGGTCCA GAGGAAGTGA

 

Quality Control:cDNA sequencing results is available upon request.