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SIRP alpha / CD172a Antibody, Rabbit PAb

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Human SIRPA Antibody Product Information
Immunogen:Recombinant Human SIRPA protein (Catalog#11612-H08H)
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Human SIRPA (rh SIRPA; Catalog#11612-H08H; NP_542970.1; Met 1-Arg 370). Total IgG was purified by Protein A affinity chromatography .
Human SIRPA Antibody Usage Guide
Specificity:Human SIRPA / CD172A
Application:ELISA

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human SIRPA. The detection limit for Human SIRPA is approximately 0.00245 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Other SIRPA Antibody Products
Reactivity: Human  
Application: ELISA  WB  FCM  
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11612-MM03-50
11612-MM03-100
11612-MM03-200
50 µg 
100 µg 
200 µg 
Add to Cart
Reactivity: Human  
Application: ELISA  IP  
  • Slide 1
11612-RP02-50
11612-RP02-100
11612-RP02-200
50 µg 
100 µg 
200 µg 
Add to Cart
Reactivity: Human  
Application: FCM  
  • Slide 1
11612-MM03-F-50
11612-MM03-F-100
50 Tests 
100 Tests 
Add to Cart
Reactivity: Human  
Application: FCM  
  • Slide 1
11612-MM03-A-100
11612-MM03-A-50
100 Tests 
50 Tests 
Add to Cart
Reactivity: Human  
Application: FCM  
  • Slide 1
11612-MM03-P-100
11612-MM03-P-100
11612-MM03-P-50
100 Tests 
100 Tests 
50 Tests 
Add to Cart
Reactivity: Mouse  
Application: FCM  
  • Slide 1
50956-R001-P-50
50956-R001-P-100
50 Tests 
100 Tests 
Add to Cart
Reactivity: Mouse  
Application: ELISA  
    50956-T16-50
    50956-T16-100
    50956-T16-200
    50 µg 
    100 µg 
    200 µg 
    Add to Cart
    Reactivity: Mouse  
    Application: ELISA  
      50956-R001-50
      50956-R001-100
      50 µg 
      100 µg 
      Add to Cart
      Reactivity: Mouse  
      Application: ELISA  
        50956-R009-50
        50956-R009-100
        50 µg 
        100 µg 
        Add to Cart
        Reactivity: Mouse  
        Application: FCM  
        • Slide 1
        50956-R001-A-50
        50956-R001-A-100
        50 Tests 
        100 Tests 
        Add to Cart
        Reactivity: Mouse  
        Application: ELISA  
          50956-RP01-200
          50956-RP01-100
          50956-RP01-400
          200 µg 
          100 µg 
           
          Add to Cart
          Reactivity: Mouse  
          Application: FCM  
          • Slide 1
          50956-R001-F-100
          50956-R001-F-50
          100 Tests 
          50 Tests 
          Add to Cart
          Reactivity: Rat  
          Application: ELISA  WB  IHC-P  
          • Slide 1
          • Slide 1
          • Slide 1
          80270-T56-200
          80270-T56-100
          80270-T56-50
          200 µg 
          100 µg 
          50 µg 
          Add to Cart
          Reactivity: Rat  
          Application: ELISA  
            80270-RP01-400
            80270-RP01-200
            80270-RP01-100
             
            200 µg 
            100 µg 
            Add to Cart
            SIRPA/SIRP alpha/CD172a Background

            Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.

            Human SIRPA/SIRP alpha/CD172a References
          • Timms JF. et al., 1999, Curr Biol. 9: 927-30.
          • Stofega MR. et al., 2000, J Biol Chem. 275: 28222-9.
          • Liu T. et al., 2005, J Proteome Res. 4: 2070-80.
          • Wolf-Yadlin A. et al., 2007, Proc Natl Acad Sci. 104: 5860-5.
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            Catalog: 11612-RP01-100
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            200 µg
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            All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
            Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"