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SIRP alpha / CD172a Antibody, Mouse MAb

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Human SIRPA Antibody Product Information
Immunogen:Recombinant Human SIRPA protein (Catalog#11612-H08H)
Clone ID:03
Ig Type:Mouse IgG2b
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SIRPA (rh SIRPA; Catalog#11612-H08H; NP_542970.1; Met 1-Arg 370). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography .
Human SIRPA Antibody Usage Guide
Specificity:Human SIRPA / CD172A
No cross-reactivity with Human cell lysate (293 cell line) in WB and ELISA.
Application:WB, ELISA, FCM

WB: 10-20 μg/mL

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human SIRPA. The detection limit for Human SIRPA is 0.0195 ng/well.

FCM: 0.5-2 μg/Test

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human SIRPA Antibody WB Application Image
Human SIRPA Antibody FC Application Image
SIRP alpha / CD172a Antibody, Mouse MAb, Flow cytometric analysis
[Click to enlarge image]
Caption:
Flow cytometric analysis of Human SIRPα(CD172a) expression on human peripheral blood monocytes. Cells were stained with purified anti-Human SIRPα(CD172a), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of viable monocytes.
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              SIRPA/SIRP alpha/CD172a Background

              Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.

              Human SIRPA/SIRP alpha/CD172a References
            • Timms JF. et al., 1999, Curr Biol. 9: 927-30.
            • Stofega MR. et al., 2000, J Biol Chem. 275: 28222-9.
            • Liu T. et al., 2005, J Proteome Res. 4: 2070-80.
            • Wolf-Yadlin A. et al., 2007, Proc Natl Acad Sci. 104: 5860-5.
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              Catalog: 11612-MM03-50
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              All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
              Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"