Human SILV Gene cDNA Clone / ORF Clone

SILV cDNA Clone Datasheet

SILV cDNA Clone Product Information

• Gene Bank Ref.ID: BC001414
• cDNA Size: 1986
• Gene Synonym: P1;SI;SIL;ME20;P100;SILV;ME20M;gp100;ME20-M;PMEL17;D12S53E;PMEL
• Source: Human

SILV cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens silver homolog (mouse) DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

SILV cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SILV cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SILV cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGATCTGG TGCTAAAAAG ATGCCTTCTT CATTTGGCTG TGATAGGTGC TTTGCTGGCT
61       GTGGGGGCTA CAAAAGTACC CAGAAACCAG GACTGGCTTG GTGTCTCAAG GCAACTCAGA
121      ACCAAAGCCT GGAACAGGCA GCTGTATCCA GAGTGGACAG AAGCCCAGAG ACTTGACTGC
181      TGGAGAGGTG GTCAAGTGTC CCTCAAGGTC AGTAATGATG GGCCTACACT GATTGGTGCA
241      AATGCCTCCT TCTCTATTGC CTTGAACTTC CCTGGAAGCC AAAAGGTATT GCCAGATGGG
301      CAGGTTATCT GGGTCAACAA TACCATCATC AATGGGAGCC AGGTGTGGGG AGGACAGCCA
361      GTGTATCCCC AGGAAACTGA CGATGCCTGC ATCTTCCCTG ATGGTGGACC TTGCCCATCT
421      GGCTCTTGGT CTCAGAAGAG AAGCTTTGTT TATGTCTGGA AGACCTGGGG CCAATACTGG
481      CAAGTTCTAG GGGGCCCAGT GTCTGGGCTG AGCATTGGGA CAGGCAGGGC AATGCTGGGC
541      ACACACACCA TGGAAGTGAC TGTCTACCAT CGCCGGGGAT CCCGGAGCTA TGTGCCTCTT
601      GCTCATTCCA GCTCAGCCTT CACCATTACT GACCAGGTGC CTTTCTCCGT GAGCGTGTCC
661      CAGTTGCGGG CCTTGGATGG AGGGAACAAG CACTTCCTGA GAAATCAGCC TCTGACCTTT
721      GCCCTCCAGC TCCATGACCC TAGTGGCTAT CTGGCTGAAG CTGACCTCTC CTACACCTGG
781      GACTTTGGAG ACAGTAGTGG AACCCTGATC TCTCGGGCAC TTGTGGTCAC TCATACTTAC
841      CTGGAGCCTG GCCCAGTCAC TGCCCAGGTG GTCCTGCAGG CTGCCATTCC TCTCACCTCC
901      TGTGGCTCCT CCCCAGTTCC AGGCACCACA GATGGGCACA GGCCAACTGC AGAGGCCCCT
961      AACACCACAG CTGGCCAAGT GCCTACTACA GAAGTTGTGG GTACTACACC TGGTCAGGCG
1021     CCAACTGCAG AGCCCTCTGG AACCACATCT GTGCAGGTGC CAACCACTGA AGTCATAAGC
1081     ACTGCACCTG TGCAGATGCC AACTGCAGAG AGCACAGGTA TGACACCTGA GAAGGTGCCA
1141     GTTTCAGAGG TCATGGGTAC CACACTGGCA GAGATGTCAA CTCCAGAGGC TACAGGTATG
1201     ACACCTGCAG AGGTATCAAT TGTGGTGCTT TCTGGAACCA CAGCTGCACA GGTAACAACT
1261     ACAGAGTGGG TGGAGACCAC AGCTAGAGAG CTACCTATCC CTGAGCCTGA AGGTCCAGAT
1321     GCCAGCTCAA TCATGTCTAC GGAAAGTATT ACAGGTTCCC TGGGCCCCCT GCTGGATGGT
1381     ACAGCCACCT TAAGGCTGGT GAAGAGACAA GTCCCCCTGG ATTGTGTTCT GTATCGATAT
1441     GGTTCCTTTT CCGTCACCCT GGACATTGTC CAGGGTATTG AAAGTGCCGA GATCCTGCAG
1501     GCTGTGCCGT CCGGTGAGGG GGATGCATTT GAGCTGACTG TGTCCTGCCA AGGCGGGCTG
1561     CCCAAGGAAG CCTGCATGGA GATCTCATCG CCAGGGTGCC AGCCCCCTGC CCAGCGGCTG
1621     TGCCAGCCTG TGCTACCCAG CCCAGCCTGC CAGCTGGTTC TGCACCAGAT ACTGAAGGGT
1681     GGCTCGGGGA CATACTGCCT CAATGTGTCT CTGGCTGATA CCAACAGCCT GGCAGTGGTC
1741     AGCACCCAGC TTATCATGCC TGGTCAAGAA GCAGGCCTTG GGCAGGTTCC GCTGATCGTG
1801     GGCATCTTGC TGGTGTTGAT GGCTGTGGTC CTTGCATCTC TGATATATAG GCGCAGACTT
1861     ATGAAGCAAG ACTTCTCCGT ACCCCAGTTG CCACATAGCA GCAGTCACTG GCTGCGTCTA
1921     CCCCGCATCT TCTGCTCTTG TCCCATTGGT GAGAATAGCC CCCTCCTCAG TGGGCAGCAG
1981     GTCTGA

 

Quality Control:cDNA sequencing results is available upon request.