Human SIGLEC10 transcript variant 3 Gene cDNA Clone / ORF Clone

SIGLEC10 cDNA Clone Datasheet

SIGLEC10 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001171157.1
• cDNA Size: 1809
• Gene Synonym: UNQ477/PRO940, MGC126774, PRO940, SIGLEC-10, SLG2
• Source: Human

SIGLEC10 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens sialic acid binding Ig-like lectin 10, transcript variant 3 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 853 A/G resulting in the amino acid Thr substitution by Ala.

SIGLEC10 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SIGLEC10 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SIGLEC10 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTACTGC CACTGCTGCT GTCCTCGCTG CTGGGCGGGT CCCAGGCTAT GGATGGGAGA
61       TTCTGGATAC GAGTGCAGGA GTCAGTGATG GTGCCGGAGG GCCTGTGCAT CTCTGTGCCC
121      TGCTCTTTCT CCTACCCCCG ACAAGACTGG ACAGGGTCTA CCCCAGCTTA TGGCTACTGG
181      TTCAAAGCAG TGACTGAGAC AACCAAGGGT GCTCCTGTGG CCACAAACCA CCAGAGTCGA
241      GAGGTGGAAA TGAGCACCCG GGGCCGATTC CAGCTCACTG GGGATCCCGC CAAGGGGAAC
301      TGCTCCTTGG TGATCAGAGA CGCGCAGATG CAGGATGAGT CACAGTACTT CTTTCGGGTG
361      GAGAGAGGAA GCTATGTGAG ATATAATTTC ATGAACGATG GGTTCTTTCT AAAAGTAACA
421      GCCCTGACTC AGAAGCCTGA TGTCTACATC CCCGAGACCC TGGAGCCCGG GCAGCCGGTG
481      ACGGTCATCT GTGTGTTTAA CTGGGCCTTT GAGGAATGTC CACCCCCTTC TTTCTCCTGG
541      ACGGGGGCTG CCCTCTCCTC CCAAGGAACC AAACCAACGA CCTCCCACTT CTCAGTGCTC
601      AGCTTCACGC CCAGACCCCA GGACCACAAC ACCGACCTCA CCTGCCATGT GGACTTCTCC
661      AGAAAGGGTG TGAGCGCACA GAGGACCGTC CGACTCCGTG TGGCCTATGC CCCCAGAGAC
721      CTTGTTATCA GCATTTCACG TGACAACACG CCAGCCCTGG AGCCCCAGCC CCAGGGAAAT
781      GTCCCATACC TGGAAGCCCA AAAAGGCCAG TTCCTGCGGC TCCTCTGTGC TGCTGACAGC
841      CAGCCCCCTG CCGCACTGAG CTGGGTCCTG CAGAACAGAG TCCTCTCCTC GTCCCATCCC
901      TGGGGCCCTA GACCCCTGGG GCTGGAGCTG CCCGGGGTGA AGGCTGGGGA TTCAGGGCGC
961      TACACCTGCC GAGCGGAGAA CAGGCTTGGC TCCCAGCAGC GAGCCCTGGA CCTCTCTGTG
1021     CAGTATCCTC CAGAGAACCT GAGAGTGATG GTTTCCCAAG CAAACAGGAC AGTCCTGGAA
1081     AACCTTGGGA ACGGCACGTC TCTCCCAGTA CTGGAGGGCC AAAGCCTGTG CCTGGTCTGT
1141     GTCACACACA GCAGCCCCCC AGCCAGGCTG AGCTGGACCC AGAGGGGACA GGTTCTGAGC
1201     CCCTCCCAGC CCTCAGACCC CGGGGTCCTG GAGCTGCCTC GGGTTCAAGT GGAGCACGAA
1261     GGAGAGTTCA CCTGCCACGC TCGGCACCCA CTGGGCTCCC AGCACGTCTC TCTCAGCCTC
1321     TCCGTGCACT ATAAGAAGGG ACTCATCTCA ACGGCATTCT CCAACGGAGC GTTTCTGGGA
1381     ATCGGCATCA CGGCTCTTCT TTTCCTCTGC CTGGCCCTGA TCATCATGAA GATTCTACCG
1441     AAGAGACGGA CTCAGACAGA AACCCCGAGG CCCAGGTTCT CCCGGCACAG CACGATCCTG
1501     GATTACATCA ATGTGGTCCC GACGGCTGGC CCCCTGGCTC AGAAGCGGAA TCAGAAAGCC
1561     ACACCAAACA GTCCTCGGAC CCCTCTTCCA CCAGGTGCTC CCTCCCCAGA ATCAAAGAAG
1621     AACCAGAAAA AGCAGTATCA GTTGCCCAGT TTCCCAGAAC CCAAATCATC CACTCAAGCC
1681     CCAGAATCCC AGGAGAGCCA AGAGGAGCTC CATTATGCCA CGCTCAACTT CCCAGGCGTC
1741     AGACCCAGGC CTGAGGCCCG GATGCCCAAG GGCACCCAGG CGGATTATGC AGAAGTCAAG
1801     TTCCAATGA

 

Quality Control:cDNA sequencing results is available upon request.