SIAE / AIS6 Protein (His Tag)

Sialic acid acetylesterase Protein Datasheet

SIAE / AIS6 Protein Product Information

• Synonym: SIAE, YSG2
• Protein Construction: A DNA sequence encoding the human SIAE ( Met 1- Lys523) (Q9HAT2-1) was expressed, with a C-terminal polyhistidine tag.
• Source: Human
• Expression Host: Baculovirus-Insect cells

SIAE / AIS6 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ile 24
• Molecular Mass: The secreted recombinant human SIAE consists of 511 amino acids and predicts a molecular mass of 57.4 KDa. The apparent molecular mass of the protein is approximately 61 Kda in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH8.0.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: SIAE / AIS6 protein

SIAE / AIS6 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SIAE / AIS6 Protein Description

Sialate O-acetylesterase is a member of the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is N-acyl-O-acetylneuraminate O-acetylhydrolase. Other names in common use include N-acetylneuraminate acetyltransferase, sialate 9(4)-O-acetylesterase, and sialidase. Sialate O-acetylesterase is abundantly expressed in the testis, prostate, and colon. Sialate O-acetylesterase has substrates of this enzyme. They are N-acetyl-O-acetylneuraminate and H2O, whereas its two products are N-acetylneuraminate and acetate. Sialate O-acetylesterase catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid. Defects in Sialate O-acetylesterase can cause autoimmune disease type 6 (AIS6).

References

1. Mandal C. et al., 2012, Glycobiology. 22 (1): 70-83.
2. Tsai S. et al., 2011, Placenta. 32 (2): 175-82.
3. Surolia I. et al., 2010, Nature. 466 (7303): 243-7.