Human SHMT2 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13426-G	Cloning vector ($195) pCMV / hygro ($395) pCMV / hygro - HA ($395) pCMV / hygro - His ($395) pCMV / hygro - Myc ($395) pCMV / hygro - FLAG ($395)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

SHMT2 cDNA Clone Datasheet

SHMT2 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_005412.5
cDNA Size:	1515
Gene Synonym:	GLYA; SHMT; SHMT2
Source:	Human

SHMT2 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens serine hydroxymethyltransferase 2 (mitochondrial) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

SHMT2 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SHMT2 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SHMT2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGTACT TCTCTTTGTT TTGGGCGGCT CGGCCTCTGC AGAGATGTGG GCAGCTGGTC
61       AGGATGGCCA TTCGGGCTCA GCACAGCAAC GCAGCCCAGA CTCAGACTGG GGAAGCAAAC
121      AGGGGCTGGA CAGGCCAGGA GAGCCTGTCG GACAGTGATC CTGAGATGTG GGAGTTGCTG
181      CAGAGGGAGA AGGACAGGCA GTGTCGTGGC CTGGAGCTCA TTGCCTCAGA GAACTTCTGC
241      AGCCGAGCTG CGCTGGAGGC CCTGGGGTCC TGTCTGAACA ACAAGTACTC GGAGGGTTAT
301      CCTGGCAAGA GATACTATGG GGGAGCAGAG GTGGTGGATG AAATTGAGCT GCTGTGCCAG
361      CGCCGGGCCT TGGAAGCCTT TGACCTGGAT CCTGCACAGT GGGGAGTCAA TGTCCAGCCC
421      TACTCCGGGT CCCCAGCCAA CCTGGCCGTC TACACAGCCC TTCTGCAACC TCACGACCGG
481      ATCATGGGGC TGGACCTGCC CGATGGGGGC CATCTCACCC ACGGCTACAT GTCTGACGTC
541      AAGCGGATAT CAGCCACGTC CATCTTCTTC GAGTCTATGC CCTATAAGCT CAACCCCAAA
601      ACTGGCCTCA TTGACTACAA CCAGCTGGCA CTGACTGCTC GACTTTTCCG GCCACGGCTC
661      ATCATAGCTG GCACCAGCGC CTATGCTCGC CTCATTGACT ACGCCCGCAT GAGAGAGGTG
721      TGTGATGAAG TCAAAGCACA CCTGCTGGCA GACATGGCCC ACATCAGTGG CCTGGTGGCT
781      GCCAAGGTGA TTCCCTCGCC TTTCAAGCAC GCGGACATCG TCACCACCAC TACTCACAAG
841      ACTCTTCGAG GGGCCAGGTC AGGGCTCATC TTCTACCGGA AAGGGGTGAA GGCTGTGGAC
901      CCCAAGACTG GCCGGGAGAT CCCTTACACA TTTGAGGACC GAATCAACTT TGCCGTGTTC
961      CCATCCCTGC AGGGGGGCCC CCACAATCAT GCCATTGCTG CAGTAGCTGT GGCCCTAAAG
1021     CAGGCCTGCA CCCCCATGTT CCGGGAGTAC TCCCTGCAGG TTCTGAAGAA TGCTCGGGCC
1081     ATGGCAGATG CCCTGCTAGA GCGAGGCTAC TCACTGGTAT CAGGTGGTAC TGACAACCAC
1141     CTGGTGCTGG TGGACCTGCG GCCCAAGGGC CTGGATGGAG CTCGGGCTGA GCGGGTGCTA
1201     GAGCTTGTAT CCATCACTGC CAACAAGAAC ACCTGTCCTG GAGACCGAAG TGCCATCACA
1261     CCGGGCGGCC TGCGGCTTGG GGCCCCAGCC TTAACTTCTC GACAGTTCCG TGAGGATGAC
1321     TTCCGGAGAG TTGTGGACTT TATAGATGAA GGGGTCAACA TTGGCTTAGA GGTGAAGAGC
1381     AAGACTGCCA AGCTCCAGGA TTTCAAATCC TTCCTGCTTA AGGACTCAGA AACAAGTCAG
1441     CGTCTGGCCA ACCTCAGGCA ACGGGTGGAG CAGTTTGCCA GGGCCTTCCC CATGCCTGGT
1501     TTTGATGAGC ATTGA

Quality Control:cDNA sequencing results is available upon request.