Human SHMT1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13525-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

SHMT1 cDNA Clone Datasheet

SHMT1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_148918.1
cDNA Size:	1335
Gene Synonym:	SHMT; CSHMT；SHMT1
Source:	Human

SHMT1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens serine hydroxymethyltransferase 1 (soluble) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

SHMT1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SHMT1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SHMT1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACGATGC CAGTCAACGG GGCCCACAAG GATGCTGACC TGTGGTCCTC ACATGACAAG
61       ATGCTGGCAC AACCCCTCAA AGACAGTGAT GTTGAGGTTT ACAACATCAT TAAGAAGGAG
121      AGTAACCGGC AGAGGGTTGG ATTGGAGCTG ATTGCCTCGG AGAATTTCGC CAGCCGAGCA
181      GTTTTGGAGG CCCTAGGCTC TTGCTTAAAT AACAAATACT CTGAGGGGTA CCCGGGCCAG
241      AGATACTATG GCGGGACTGA GTTTATTGAT GAACTGGAGA CCCTCTGTCA GAAGCGAGCC
301      CTGCAGGCCT ATAAGCTGGA CCCACAGTGC TGGGGGGTCA ACGTCCAGCC CTACTCAGGC
361      TCCCCTGCAA ACTTTGCTGT GTACACTGCC CTGGTGGAAC CCCATGGGCG CATCATGGGC
421      CTGGACCTTC CGGATGGGGG CCACCTGACC CATGGGTTCA TGACAGACAA GAAGAAAATC
481      TCTGCCACGT CCATCTTCTT TGAATCTATG CCCTACAAGG TGAACCCAGA TACTGGCTAC
541      ATCAACTATG ACCAGCTGGA GGAGAACGCA CGCCTCTTCC ACCCGAAGCT GATCATCGCA
601      GGAACCAGCT GCTACTCCCG AAACCTGGAA TATGCCCGGC TACGGAAGAT TGCAGATGAG
661      AACGGGGCGT ATCTCATGGC GGACATGGCT CACATCAGCG GGCTGGTGGC GGCTGGCGTG
721      GTGCCCTCCC CATTTGAACA CTGCCATGTG GTGACCACCA CCACTCACAA GACCCTGCGA
781      GGCTGCCGAG CTGGCATGAT CTTCTACAGG AAAGGGGTTG CTGTGGCACT GAAGCAAGCT
841      ATGACTCTGG AATTTAAAGT TTATCAACAC CAGGTGGTGG CCAACTGCAG GGCTCTGTCT
901      GAGGCCCTGA CGGAGCTGGG CTACAAAATA GTCACAGGTG GTTCTGACAA CCATTTGATC
961      CTTGTGGATC TCCGTTCCAA AGGCACAGAT GGTGGAAGGG CTGAGAAGGT GCTAGAAGCC
1021     TGTTCTATTG CCTGCAACAA GAACACCTGT CCAGGTGACA GAAGCGCTCT GCGGCCCAGT
1081     GGACTGCGGC TGGGGACCCC AGCACTGACG TCCCGTGGAC TTTTGGAAAA AGACTTCCAA
1141     AAAGTAGCCC ACTTTATTCA CAGAGGGATA GAGCTGACCC TGCAGATCCA GAGCGACACT
1201     GGTGTCAGAG CCACCCTGAA AGAGTTCAAG GAGAGACTGG CAGGGGATAA GTACCAGGCG
1261     GCCGTGCAGG CTCTCCGGGA GGAGGTTGAG AGCTTCGCCT CTCTCTTCCC TCTGCCTGGC
1321     CTGCCTGACT TCTAA

Quality Control:cDNA sequencing results is available upon request.