Human SHISA3 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13554-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

SHISA3 cDNA Clone Datasheet

SHISA3 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001080505.1
cDNA Size:	717
Gene Synonym:	hShisa3；SHISA3
Source:	Human

SHISA3 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens shisa homolog 3 (Xenopus laevis) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 39 G/T and 193 T/C resulting in the amino acid Trp substitution by Cys，Cys substitution by Arg.

SHISA3 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SHISA3 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SHISA3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGGGCAC TGCTGGCGCT TTGCCTTCTC CTTGGCTGTC TGCGCTGGGG CCCGGCGGGC
61       GCCCAGCAGT CCGGAGAGTA CTGCCACGGC TGGGTGGACG TGCAGGGCAA CTACCACGAG
121      GGCTTCCAGT GCCCAGAGGA CTTCGACACG CTGGACGCTA CCATCTGCTG CGGCTCCTGC
181      GCGCTCCGCT ACCGTTGCGC CGCGGCCGAC GCCAGGCTGG AGCAGGGCGG CTGCACCAAC
241      GACCGCCGCG AACTGGAGCA CCCAGGCATC ACTGCGCAGC CTGTCTACGT CCCCTTTCTC
301      ATCGTCGGCT CCATCTTCAT TGCGTTCATC ATCCTGGGCT CTGTAGTGGC TATTTATTGT
361      TGCACCTGTT TGAGACCCAA GGAGCCCTCG CAGCAGCCAA TCCGCTTCTC ACTCCGCAGC
421      TATCAGACAG AGACCCTGCC CATGATCCTG ACCTCCACCA GCCCCAGGGC ACCCTCCCGG
481      CAGTCCAGCA CAGCCACGAG CTCCAGCTCC ACAGGCGGCT CCATCCGCAG GTTCTCCTTT
541      GCCAGGGCTG AGCCGGGCTG CCTGGTGCCC TCACCGCCCC CGCCATACAC CACCAGCCAC
601      TCAATCCACC TGGCTCAGCC ATCTGGTTTC CTGGTGTCAC CCCAGTATTT CGCTTACCCC
661      CTCCAGCAGG AGCCCCCACT GCCTGGGAAG AGCTGTCCAG ACTTCAGTTC CAGTTGA

Quality Control:cDNA sequencing results is available upon request.