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Mouse SHH Gene cDNA Clone (full-length ORF Clone)
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Mouse SHH Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
MG51100-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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SHH cDNA Clone Datasheet

 

SHH cDNA Clone Product Information

Gene Bank Ref.ID: NM_009170.3

cDNA Size:

1314

Gene Synonym:

Hx; Dsh; Hhg1; Hxl3; M100081; 9530036O11Rik

Source: Mouse

SHH cDNA Clone Description

cDNA Description:

ORF Clone of Mus musculus sonic hedgehog DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 417 T/C not
causing the amino acid variation.

SHH cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SHH cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SHH cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGCTGC TGCTGGCCAG ATGTTTTCTG GTGATCCTTG CTTCCTCGCT GCTGGTGTGC
61       CCCGGGCTGG CCTGTGGGCC CGGCAGGGGG TTTGGAAAGA GGCGGCACCC CAAAAAGCTG
121      ACCCCTTTAG CCTACAAGCA GTTTATTCCC AACGTAGCCG AGAAGACCCT AGGGGCCAGC
181      GGCAGATATG AAGGGAAGAT CACAAGAAAC TCCGAACGAT TTAAGGAACT CACCCCCAAT
241      TACAACCCCG ACATCATATT TAAGGATGAG GAAAACACGG GAGCAGACCG GCTGATGACT
301      CAGAGGTGCA AAGACAAGTT AAATGCCTTG GCCATCTCTG TGATGAACCA GTGGCCTGGA
361      GTGAAGCTGC GAGTGACCGA GGGCTGGGAT GAGGACGGCC ATCATTCAGA GGAGTCCCTA
421      CACTATGAGG GTCGAGCAGT GGACATCACC ACGTCCGACC GGGACCGCAG CAAGTACGGC
481      ATGCTGGCTC GCCTGGCTGT GGAAGCAGGT TTCGACTGGG TCTACTATGA ATCCAAAGCT
541      CACATCCACT GTTCTGTGAA AGCAGAGAAC TCCGTGGCGG CCAAATCCGG CGGCTGTTTC
601      CCGGGATCCG CCACCGTGCA CCTGGAGCAG GGCGGCACCA AGCTGGTGAA GGACTTACGT
661      CCCGGAGACC GCGTGCTGGC GGCTGACGAC CAGGGCCGGC TGCTGTACAG CGACTTCCTC
721      ACCTTCCTGG ACCGCGACGA AGGCGCCAAG AAGGTCTTCT ACGTGATCGA GACGCTGGAG
781      CCGCGCGAGC GCCTGCTGCT CACCGCCGCG CACCTGCTCT TCGTGGCGCC GCACAACGAC
841      TCGGGGCCCA CGCCCGGGCC AAGCGCGCTC TTTGCCAGCC GCGTGCGCCC CGGGCAGCGC
901      GTGTACGTGG TGGCTGAACG CGGCGGGGAC CGCCGGCTGC TGCCCGCCGC GGTGCACAGC
961      GTGACGCTGC GAGAGGAGGA GGCGGGCGCG TACGCGCCGC TCACGGCGCA CGGCACCATT
1021     CTCATCAACC GGGTGCTCGC CTCGTGCTAC GCTGTCATCG AGGAGCACAG CTGGGCACAC
1081     CGGGCCTTCG CGCCTTTCCG CCTGGCGCAC GCGCTGCTGG CCGCGCTGGC ACCCGCCCGC
1141     ACGGACGGCG GGGGCGGGGG CAGCATCCCT GCAGCGCAAT CTGCAACGGA AGCGAGGGGC
1201     GCGGAGCCGA CTGCGGGCAT CCACTGGTAC TCGCAGCTGC TCTACCACAT TGGCACCTGG
1261     CTGTTGGACA GCGAGACCAT GCATCCCTTG GGAATGGCGG TCAAGTCCAG CTGA

 

Quality Control:cDNA sequencing results is available upon request.

 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"