Human SHARPIN Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13413-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

SHARPIN cDNA Clone Datasheet

SHARPIN cDNA Clone Product Information

Gene Bank Ref.ID:	NM_030974.3
cDNA Size:	1164
Gene Synonym:	SIPL1; SHARPIN
Source:	Human

SHARPIN cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens SHANK-associated RH domain interactor DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 154 C/A not causing the amino acid variation.

SHARPIN cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SHARPIN cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SHARPIN cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGCCGC CAGCGGGCGG GGCGGCGGCG GCGGCCTCGG ACTTGGGCTC CGCCGCAGTG
61       CTCTTGGCTG TGCACGCCGC GGTGAGGCCG CTGGGCGCCG GGCCAGACGC CGAGGCACAG
121      CTGCGGAGGC TGCAGCTGAG CGCGGACCCT GAGAGGCCTG GGCGCTTCCG GCTGGAGCTG
181      CTGGGCGCGG GACCTGGGGC GGTTAATTTG GAGTGGCCCC TGGAGTCAGT TTCCTACACC
241      ATCCGAGGCC CCACCCAGCA CGAGCTACAG CCTCCACCAG GAGGGCCTGG AACCCTCAGC
301      CTGCACTTCC TCAACCCTCA GGAAGCTCAG CGGTGGGCAG TCCTAGTCCG AGGTGCCACC
361      GTGGAAGGAC AGAATGGCAG CAAGAGCAAC TCACCACCAG CCTTGGGCCC AGAAGCATGC
421      CCTGTCTCCC TGCCCAGTCC CCCGGAAGCC TCCACACTCA AGGGCCCTCC ACCTGAGGCA
481      GATCTTCCTA GGAGCCCTGG AAACTTGACG GAGAGAGAAG AGCTGGCAGG GAGCCTGGCC
541      CGGGCTATTG CAGGTGGAGA CGAGAAGGGG GCAGCCCAAG TGGCAGCCGT CCTGGCCCAG
601      CATCGTGTGG CCCTGAGTGT TCAGCTTCAG GAGGCCTGCT TCCCACCTGG CCCCATCAGG
661      CTGCAGGTCA CACTTGAAGA CGCTGCCTCT GCCGCATCCG CCGCGTCCTC TGCACACGTT
721      GCCCTGCAGG TCCACCCCCA CTGCACTGTT GCAGCTCTCC AGGAGCAGGT GTTCTCAGAG
781      CTCGGTTTCC CGCCAGCCGT GCAACGCTGG GTCATCGGAC GGTGCCTGTG TGTGCCTGAG
841      CGCAGCCTTG CCTCTTACGG GGTTCGGCAG GATGGGGACC CTGCTTTCCT CTACTTGCTG
901      TCAGCTCCTC GAGAAGCCCC AGCCACAGGA CCTAGCCCTC AGCACCCCCA GAAGATGGAC
961      GGGGAACTTG GACGCTTGTT TCCCCCATCA TTGGGGCTAC CCCCAGGCCC CCAGCCAGCT
1021     GCCTCCAGCC TGCCCAGTCC ACTCCAGCCC AGCTGGTCCT GTCCTTCCTG CACCTTCATC
1081     AATGCCCCAG ACCGCCCTGG CTGTGAGATG TGTAGCACCC AGAGGCCCTG CACTTGGGAC
1141     CCCCTTGCTG CAGCTTCCAC CTAG

Quality Control:cDNA sequencing results is available upon request.