SH2D1A Protein (His Tag)

SH2 domain containing 1A Protein Datasheet

SH2D1A Protein Product Information

• Synonym: RP5-1052M9.3, DSHP, EBVS, FLJ18687, FLJ92177, IMD5, LYP, MTCP1, SAP, SAP/SH2D1A, XLP, XLPD
• Protein Construction: A DNA sequence encoding the human SH2D1A isoform 1 (NP_002342.1) (Pro 2-Lys 97) was expressed, with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: E. Coli

SH2D1A Protein QC Testing

• Purity: > 94 % as determined by SDS-PAGE.
• Bio-activity: 1. Measured by its ability to bind recombinant human SLAMF1 in a functional ELISA
  2. Measured by its ability to bind recombinant human SLAMF6 in a functional ELISA
  3. Measured by its ability to bind recombinant mouse SLAMF6 in a functional ELISA
  4. Measured by its ability to bind recombinant mouse CD84 in a functional ELISA
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human SH2D1A consisting of 139 amino acids and has a calculated molecular mass of 15.6 kDa .It migrates as an approximately 14 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.5, 20% glycerol
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: SH2D1A protein

SH2D1A Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SH2D1A Protein Description

SH2 domain-containing protein 1A(SH2D1A/ SAP) is a 128 amino acid (aa) protein, containing a single Src homology 2 (SH2) domain, flanked by 5 aa at the N- and 25 aa at the C-terminus. The absence of a catalytic domain and the presence of an SH2 domain suggest that SH2D1A regulates 1 or more signal transduction pathways. It interacts with signaling lymphocytic activation molecule (SLAM) expressed on the surface of activated T and B cells. SH2D1A (SAP) interacts via its SH2 domain with a motif (TIYXXV) present in the cytoplasmic tail of the cell-surface receptors CD150/SLAM, CD84, CD229/Ly-9, and CD244/2B4. It has been found that SH2D1A interacts with signaling lymphocytic activation molecule (SLAM), a transmembrane protein that is expressed on the surface of activated T and B cells. SH2D1A was expressed in EBV-carrying, tumor phenotype representative (type I), but not in EBV- carrying lymphoblastoid cell line (LCL)-like (type III) or EBV-negative Burkitt lymphoma (BL) lines.

References

1. Morra M, et al. (2005) PNAS. 102(13): 4819-23.
2. Morra M, et al. (2001) The Journal of Biological Chemistry. 276: 36809-16.
3. Hron JD, et al. (2004) JEM. 200 (2): 261-6.