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Human SGCA Gene cDNA Clone (full-length ORF Clone)
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Human SGCA Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG13540-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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SGCA cDNA Clone Datasheet

 

SGCA cDNA Clone Product Information

Gene Bank Ref.ID: BC025702

cDNA Size:

1164

Gene Synonym:

A2;ADL;DAG2;DMDA2;50-DAG;LGMD2D;SCARMD1;adhalin;SGCA

Source: Human

SGCA cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens sarcoglycan, alpha (50kDa dystrophin-associated gl DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref.ID sequence.

SGCA cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SGCA cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SGCA cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTGAGA CACTCTTCTG GACTCCTCTC CTCGTGGTTC TCCTGGCAGG GCTGGGGGAC
61       ACCGAGGCCC AGCAGACCAC GCTACACCCA CTTGTGGGCC GTGTCTTTGT GCACACCTTG
121      GACCATGAGA CGTTTCTGAG CCTTCCTGAG CATGTCGCTG TCCCACCCGC TGTCCACATC
181      ACCTACCACG CCCACCTCCA GGGACACCCA GACCTGCCCC GGTGGCTCCG CTACACCCAG
241      CGCAGCCCCC ACCACCCTGG CTTCCTCTAC GGCTCTGCCA CCCCAGAAGA TCGTGGGCTC
301      CAGGTCATTG AGGTCACAGC CTACAATCGG GACAGCTTTG ATACCACTCG GCAGAGGCTG
361      GTGCTGGAGA TTGGGGACCC AGAAGGCCCC CTGCTGCCAT ACCAAGCCGA GTTCCTGGTG
421      CGCAGCCACG ATGCGGAGGA GGTGCTGCCC TCAACACCTG CCAGCCGCTT CCTCTCAGCC
481      TTGGGGGGAC TCTGGGAGCC CGGAGAGCTT CAGCTGCTCA ACGTCACCTC TGCCTTGGAC
541      CGTGGGGGCC GTGTCCCCCT TCCCATTGAG GGCCGAAAAG AAGGGGTATA CATTAAGGTG
601      GGTTCTGCCT CACCTTTTTC TACTTGCCTG AAGATGGTGG CATCCCCCGA TAGCCACGCC
661      CGCTGTGCCC AGGGCCAGCC TCCACTTCTG TCTTGCTACG ACACCTTGGC ACCCCACTTC
721      CGCGTTGACT GGTGCAATGT GACCCTGGTG GATAAGTCAG TGCCGGAGCC TGCAGATGAG
781      GTGCCCACCC CAGGTGATGG GATCCTGGAG CATGACCCGT TCTTCTGCCC ACCCACTGAG
841      GCCCCAGACC GTGACTTCTT GGTGGATGCT CTGGTCACCC TCCTGGTGCC CCTGCTGGTG
901      GCCCTGCTTC TCACCTTGCT GCTGGCCTAT GTCATGTGCT GCCGGCGGGA GGGAAGGCTG
961      AAGAGAGACC TGGCTACCTC CGACATCCAG ATGGTCCACC ACTGCACCAT CCACGGGAAC
1021     ACAGAGGAGC TGCGGCAGAT GGCGGCCAGC CGCGAGGTGC CCCGGCCACT CTCCACCCTG
1081     CCCATGTTCA ATGTGCACAC AGGTGAGCGG CTGCCTCCCC GCGTGGACAG CGCCCAGGTG
1141     CCCCTCATTC TGGACCAGCA CTGA

 

Quality Control:cDNA sequencing results is available upon request.

 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"