Human SERPINB3 Gene cDNA Clone / ORF Clone

SERPINB3 cDNA Clone Datasheet

SERPINB3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_006919.2
• cDNA Size: 1173
• Gene Synonym: SCC; T4-A; SCCA1; SCCA-1; HsT1196; SCCA-PD;SERPINB3
• Source: Human

SERPINB3 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens serpin peptidase inhibitor, clade B (ovalbumin), member 3 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1069 A/G resulting in the amino acid Thr substitution by Ala.

SERPINB3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SERPINB3 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SERPINB3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAATTCAC TCAGTGAAGC CAACACCAAG TTCATGTTCG ACCTGTTCCA ACAGTTCAGA
61       AAATCAAAAG AGAACAACAT CTTCTATTCC CCTATCAGCA TCACATCAGC ATTAGGGATG
121      GTCCTCTTAG GAGCCAAAGA CAACACTGCA CAACAGATTA AGAAGGTTCT TCACTTTGAT
181      CAAGTCACAG AGAACACCAC AGGAAAAGCT GCAACATATC ATGTTGATAG GTCAGGAAAT
241      GTTCATCACC AGTTTCAAAA GCTTCTGACT GAATTCAACA AATCCACTGA TGCATATGAG
301      CTGAAGATCG CCAACAAGCT CTTCGGAGAA AAAACGTATC TATTTTTACA GGAATATTTA
361      GATGCCATCA AGAAATTTTA CCAGACCAGT GTGGAATCTG TTGATTTTGC AAATGCTCCA
421      GAAGAAAGTC GAAAGAAGAT TAACTCCTGG GTGGAAAGTC AAACGAATGA AAAAATTAAA
481      AACCTAATTC CTGAAGGTAA TATTGGCAGC AATACCACAT TGGTTCTTGT GAACGCAATC
541      TATTTCAAAG GGCAGTGGGA GAAGAAATTT AATAAAGAAG ATACTAAAGA GGAAAAATTT
601      TGGCCAAACA AGAATACATA CAAGTCCATA CAGATGATGA GGCAATACAC ATCTTTTCAT
661      TTTGCCTCGC TGGAGGATGT ACAGGCCAAG GTCCTGGAAA TACCATACAA AGGCAAAGAT
721      CTAAGCATGA TTGTGTTGCT GCCAAATGAA ATCGATGGTC TCCAGAAGCT TGAAGAGAAA
781      CTCACTGCTG AGAAATTGAT GGAATGGACA AGTTTGCAGA ATATGAGAGA GACACGTGTC
841      GATTTACACT TACCTCGGTT CAAAGTGGAA GAGAGCTATG ACCTCAAGGA CACGTTGAGA
901      ACCATGGGAA TGGTGGATAT CTTCAATGGG GATGCAGACC TCTCAGGCAT GACCGGGAGC
961      CGCGGTCTCG TGCTATCTGG AGTCCTACAC AAGGCCTTTG TGGAGGTTAC AGAGGAGGGA
1021     GCAGAAGCTG CAGCTGCCAC CGCTGTAGTA GGATTCGGAT CATCACCTGC TTCAACTAAT
1081     GAAGAGTTCC ATTGTAATCA CCCTTTCCTA TTCTTCATAA GGCAAAATAA GACCAACAGC
1141     ATCCTCTTCT ATGGCAGATT CTCATCCCCG TAG

 

Quality Control:cDNA sequencing results is available upon request.