Cynomolgus monkey SEMA7A Gene cDNA Clone / ORF Clone

SEMA7A cDNA Clone Datasheet

SEMA7A cDNA Clone Product Information

• cDNA Size: 1995
• Gene Synonym: SEMA7A
• Source: Cynomolgus monkey

SEMA7A cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group) DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: No point mutation in the sequence.
  Please check the sequence information before order.

SEMA7A cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SEMA7A cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SEMA7A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACGCCTC CTCCGCCCGG ACGTGCCGCC CCCAGCGCAC CGCGCGCCCG CGTCCCTGGC
61       CCACCGGCTC GGTCGGTGCT CCTGCTGCGG CTGCTGCTGC TGCTCTGGGC GGCCGCCGCC
121      TCCGCCCAAG GCCACTCAAG GAGCGGACCC CGCATCTTCG CCGTCTGGAA AGGCCATGTA
181      GGGCAGGACC GGGTGGACTT TGGCCAGACT GAGCCGCACA CGGTGCTTTT CCATGAGCCA
241      GGCAGCTCCT CCGTGTGGGT GGGAGGACGC GGCAAGGTCT ACCTCTTTGA TTTCCCCGAG
301      GGCAAGAACG CATCTGTGCG CACGGTGAAC ATTGGCTCCA CAAAGGGGTC CTGTCTGGAT
361      AAGCGGGACT GCGAGAACTA CATCACTCTC CTGGAGAGGC GGAGTGAGGG GCTGCTGGCC
421      TGTGGCACCA ATGCCCGGCA CCCCAGCTGC TGGAACCTGG TGAATGACAC TGTGGTGCCA
481      CTTGGCGAGA TGAGAGGCTA TGCCCCCTTC AGCCCGGATG AGAACTCCCT GGTTCTGTTT
541      GAAGGGGACG AGGTGTATTC CACCATCCGG AAGCAGGAAT ACAATGGGAA GATCCCTCGG
601      TTCCGCCGCA TCCGGGGCGA GAGTGAGCTG TACACCAGTG ATACTGTCAT GCAGAACCCA
661      CAGTTCATCA AAGCCACCAT CGTGCACCAA GACCAGGCTT ACGATGACAA GATCTACTAC
721      TTCTTCCGAG AGGACAATCC TGACAAGAAT CCTGAGGCTC CTCTCAATGT GTCCCGTGTG
781      GCCCAGCTGT GCAGGGGGGA CCAGGGTGGG GAAAGCTCAC TGTCAGTGTC CAAGTGGAAC
841      ACTTTTCTGA AAGCCATGCT GGTATGCAGT GACGCTGCCA CCAACAAGAA CTTCAACAGG
901      CTACAAGACG TCTTCCTGCT CCCTGACCCC AGCGGCCAGT GGAGAGACAC CAGGGTCTAT
961      GGTGTTTTCT CCAACCCATG GAACTACTCA GCGGTCTGCG TGTATTCCCT CGGTGATATT
1021     GACAAGGTCT TCCGCACCTC CTCACTCAAG GGCTACCACT CAAGCCTTCC CAACCCTCGG
1081     CCTGGCAAGT GCCTCCCAGA CCAGCAGCCG ATACCCACAG AGACCTTCCA GGTGGCTGAC
1141     CGTCACCCAG AGGTGGCGCA GAGGGTGGAG CCTATGGGGC CCCTGAAGAC GCCATTGTTC
1201     CACTCTAAAT ACCACTACCA GAAAGTGGTC GTCCACCGCA TGCAAGCCAG CCACGGGGAG
1261     ACCTATCATG TGCTTTACCT AACTACAGAC AGGGGCACCA TCCACAAGGT GGTGGAGCCG
1321     GGGGAGCAGG AGCACAGCTT CATCTTCAAC ATCATGGAGA TCCAGCCCTT CCGCCGCGCA
1381     GCTGCCATCC AGACCATGTC GCTGGATGCT GAGCGGAGGA AGCTGTATGT GAGCTCCCAG
1441     TGGGAGGTGA GCCAGGTGCC CCTGGACCTG TGTGAAGTCT ATGGCGGGGG CTGCCACGGT
1501     TGCCTCATGT CCAGAGACCC CTACTGCGGC TGGGACCAGG GCCGCTGCGT CTCCATCTAC
1561     AGTTCCCAAC GGTCAGTGCT GCAATCCATT AATCCAGTTG AGCCACACAA GGAGTGTCCC
1621     AACCCCAAAC CAGACAAGGC CCCACTGCAG AAGGTTTCCC TGGCCCCGAA CTCTCGCTAC
1681     TACCTGAGCT GCCCTATGGA ATCCCGCCAC GCCACCTACT CATGGCGCCA CAAGGAGAAC
1741     GTGGAGCAGA GCTGCGAACC TGGTCACCAG AGCCCCAACT GCATCTTGTT CATCGAGAAC
1801     CTCACGGCGC AGCAGTACGG CCACTACTTC TGTGAGGCCC AGGAGGGCTC CTACTTCCGC
1861     GAGGCTCAGC ACTGGCAGCT GCTGCCCGAG GATGGCATCA TGGCTGAGCA CCTGCTGGGC
1921     CATGCCTGTG CCCTGGCCGC CTCCCTCTGG CTGGGGGTCC TGCCCACACT CACTCTTGGC
1981     TTGCTGGTCC ACTAG

 

Quality Control:cDNA sequencing results is available upon request.