Rat SEMA7A Gene cDNA Clone / ORF Clone

SEMA7A cDNA Clone Datasheet

SEMA7A cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001108153.1
• cDNA Size: 2001
• Gene Synonym: Sema7a
• Source: Rat

SEMA7A cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1263 C/T not causing the amino acid variation.

SEMA7A cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SEMA7A cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SEMA7A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACGCCTC CTCCTCCCGG ACGTGCCGCC CCCAGCGCAC CGCGCGCCCG CGTCCTCAGC
61       CTGCCGGCTC GGTTCGGGCT CCCGCTGCGG CTGCGGCTGC TGCTGGTGTT CTGGGTGACC
121      GCCGCCTCCG CCCAAGGCCA CTCGAGGAGC GGACCCCGTA TCTCCGCCGT CTGGAAAGGG
181      CAGGACCATG TGGACTTTGG CCAGCCTGAG CCACACAATG TGCTTTTCCA TGAGCCGGGC
241      AGCTTCTCTG TCTGGGTAGG TGGACGAGGC AAGGTCTACC TCTTCAACTT CCCCGAGGGC
301      AAGAATGCCT CTGTGCGCAC GGTGAACATT GGCTCCACAA AAGGGTCCTG TCAGGACAAA
361      CAGGACTGCG AGAATTACAT CACTCTTCTT GAGAGGCGGG GTAATGGACT GCTGGTCTGT
421      GGCACCAATG CCCGGAAACC CAGCTGCTGG AACTTGGTGA ATGACAGTGT GGTGATGTCA
481      CTTGGTGAGA TGAAAGGCTA TGCCCCCTTC AGCCCGGATG AGAACTCCCT GGTTCTGTTT
541      GAAGGGGATG AAGTGTACTC TACAATCCGG AAGCAGGAAT ACAATGGCAA GATCCCTCGC
601      TTTCGCCGCA TTCGGGGAGA GAGTGAACTG TACACAAGTG ATACTGTCAT GCAGAATCCA
661      CAGTTCATCA AGGCCACCAT TGTGCACCAG GACCAAGCCT ATGATGATAA GATCTACTAC
721      TTCTTCCGAG AAGACAACCC TGACAAGAAC CCCGAGGCTC CGCTCAATGT ATCCCGAGTG
781      GCCCAGTTAT GCAGGGGGGA CCAGGGTGGT GAGAGTTCAT TGTCTGTCTC CAAGTGGAAC
841      ACCTTCCTGA AAGCCATGCT GGTTTGCAGC GACGCAGCCA CCAACAGGAA CTTCAATCGA
901      CTGCAAGATG TCTTCCTACT CCCTGACCCC AGTGGCCAGT GGAGGGATAC CAGGGTCTAT
961      GGCGTTTTCT CCAACCCCTG GAACTACTCA GCTGTCTGCG TGTATTCGCT TGGTGACATT
1021     GACAGAGTCT TCCGTACCTC CTCACTCAAA GGCTACCACA TGGGCCTTCC CAACCCTCGA
1081     CCTGGCATGT GCCTCCCAAA AAAGCAACCC ATACCCACGG AAACCTTCCA GGTAGCCGAC
1141     AGTCACCCGG AGGTGGCCCA GAGAGTGGAA CCCATGGGGC CACTGAAGAC ACCATTGTTC
1201     CACTCTAAGT ACCATTACCA GAAAGTGGTT GTCCACCGCA TGCAAGCCAG CAATGGGGAG
1261     ACTTTCCATG TACTTTACCT AACCACAGAC AGGGGCACCA TTCACAAGGT GGTGGAATCA
1321     GGGGACCAGG AACACAGCTT TGTCTTCAAC ATCATGGAGA TCCAGCCCTT TCACCGTGCA
1381     GCTGCCATCC AGGCCATATC GTTGGATGCT GACCGGCGGA AGCTCTATGT GACCTCCCCG
1441     TGGGAAGTGA GCCAGGTACC CCTGGACATG TGTGGGGTCT ACAGTGGGGG CTGCCATGGC
1501     TGCCTCATGT CCCGAGACCC CTACTGTGGC TGGGACCAGG ACCAGGACCG CTGTGTGTCT
1561     ATCTACAGCT CCCAACGGTC AGTGCTGCAG TCCATCAATC CAGCTGAGCC ACACAGAGAG
1621     TGTCCCAACC CCAACCCAGA TGAGGCTCCA TTGCAGAAGG TTTCCCTGGC CCGGAACTCT
1681     CGATACTACC TGACCTGCCC CATGGAGTCC CGCCACGCCA CCTACTTATG GCGCCACGAG
1741     GAGAATGTGG AACAGAGCTG TGAGCCAGGC CACCAAAGCC CCAGCTGCAT TCTATTCATA
1801     GAGAACCTCA CTGCCCGTCA GTACGGCCAC TACCGCTGCG AGGCCCAAGA GGGTTCCTAC
1861     CTCCATGAGG CTCAACACTG GGAGCTGCTG CCGGAGGACA GAGCGCTGGC CGAACAGCTC
1921     TTGGGCCATG CCCGGGCCCT GGCCGCCTCC TTCTGGCTGG GGGTCCTGCC CACACTCGTA
1981     CTAGGTCTGC TGGTTCACTA G

 

Quality Control:cDNA sequencing results is available upon request.