Mouse SEMA5A / Semaphorin-5A / SEMAF Protein (His Tag)

sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A Protein Datasheet

SEMA5A / Semaphorin-5A / SEMAF Protein Product Information

• Synonym: SEMA5A
• Protein Construction: A DNA sequence encoding the mouse SEMA5A (Q3UPZ0) (Met1-Thr765) was expressed with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Human Cells

SEMA5A / Semaphorin-5A / SEMAF Protein QC Testing

• Purity: > 85% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Pro 22
• Molecular Mass: The recombinant mouse SEMA5A comprises 755 amino acids and has a predicted molecular mass of 85.1 kDa. The apparent molecular mass of the protein is approximately 96 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: SEMA5A / Semaphorin-5A / SEMAF protein

SEMA5A / Semaphorin-5A / SEMAF Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SEMA5A / Semaphorin-5A / SEMAF Protein Description

Semaphorins are secreted, transmembrane, and GPI-linked proteins, defined by cysteine-rich semaphorin protein domains, that have important roles in a variety of tissues. Humans have 20 semaphorins, Drosophila has five, and two are known from DNA viruses. Semaphorins are found in nematodes and crustaceans but not in non-animals. They are grouped into eight classes on the basis of phylogenetic tree analyses and the presence of additional protein motifs. Semaphorins have been implicated in diverse developmental processes such as axon guidance during nervous system development and regulation of cell migration. Semaphorin-5A, also known as Semaphorin-F, Sema F, SEMA5A and SEMAF, is a single-pass type I membrane protein which belongs to the semaphorin family. Semaphorin5A / SEMA5A contains one PSI domain, one Sema domain and seven TSP type-1 domains. It may act as positive axonal guidance cues. Semaphorin5A / SEMA5A is an axon regulator molecule and plays major roles during neuronal and vascular development. It plays an essential role in embryonic development. Semaphorin5A / SEMA5A induces endothelial cell migration from pre-existing vessels. It also plays a role in autism, reducing the ability of neurons to form connections with other neurons in certain brain regions.

References

1. Strausberg RL. et al., 2003, Proc Natl Acad Sci.  99 (26): 16899-903.
2. Neufeld G. et al., 2005, Front Biosci. 10: 751-60.
3. Fiore R. et al., 2005, Mol Cell Biol. 25 (6): 2310-9.
4. Yazdani U. et al., 2006, Genome Biol. 7 (3): 211.
5. Sadanandam A. et al., 2010, Microvasc Res. 79 (1): 1-9.