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Human SEMA4G Gene cDNA Clone (full-length ORF Clone)
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Human SEMA4G Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG12221-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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SEMA4G cDNA Clone Datasheet

 

SEMA4G cDNA Clone Product Information

Gene Bank Ref.ID: BC020960

cDNA Size:

384

Gene Synonym:

SEMA4G

Source: Human

SEMA4G cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens sema domain, immunoglobulin domain (Ig), transmemb DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref.ID sequence.

SEMA4G cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SEMA4G cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SEMA4G cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCTCAA TGTCACCACC CTCTGCATGG CCCTGTGTGC TGGATGGTCC TGAAACCAGA
61       CAAGACCTCT GCCAGCCACC TAAGCCCTGC GTACATTCAC ATGCACACAT GGAAGAATGT
121      TTATCGGCTG GGCTGCAGTG CCCCCACCCT CACCTTCTCC TGGTGCATTC TTGTTTCATC
181      CCTGCTTCTG GACTTGGGGT ACCCTCCCAA TTGCCACATC CTATCTGGTC CTCTTCCCCA
241      GCCCCATGTG GTGACCTCTT TGTCAAGAGC TTGGGAACGG GCCAGCCTGG GGAGGTAAGA
301      CTGCATCACT CCCCTCCTCT CCCTTCCTGT GTGGCCCTTG TGAATCAGCC TCCCCACTCT
361      CCTTGGTCAT TCTCAAGAGT ATGA

 

Quality Control:cDNA sequencing results is available upon request.

 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"