Cynomolgus monkey SEMA4D Gene cDNA Clone / ORF Clone

SEMA4D cDNA Clone Datasheet

SEMA4D cDNA Clone Product Information

• cDNA Size: 2589
• Gene Synonym: SEMA4D
• Source: Cynomolgus monkey

SEMA4D cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 2048 T/C, 995 C/T resulting in the amino acid Leu substitution by Pro, Thr substitution by Ile and 825 T/G, 1227 T/C, 1788 G/T not causing the amino acid variation.
  Please check the sequence information before order.

SEMA4D cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SEMA4D cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SEMA4D cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGGATGT GCATCCCTAT TAGGGGGCTG CTCATGGCCC TTGCAGTGAT GTTTGGGACA
61       GCGATGGCTT TTGCACCCAT ACCCCGGATC ACCTGGGAGC ACAGAGAGGT GCGCCTGGTG
121      CAGTTTCACG AGCCGGACAT CTACAACTAC TCAGCCTTGC TGCTGAGCGA GGACAAGGAC
181      ACCTTGTACA TAGGCGCCCG GGAGGCGGTC TTCGCTGTGA ACGCACTCAA CATCTCCGAG
241      AAGCAGCATG AGGTGTATTG GAAGGTCTCA GAAGACAAAA AAGCAAAATG TGCAGAAAAG
301      GGGAAATCAA AACAGACAGA GTGCCTCAAC TACATCCGGG TGCTGCAGCC GCTCAGCGCC
361      ACTTCCCTTT ACGTGTGTGG GACCAACGCA TTCCAGCCGG CCTGTGACCA CCTGAACTTA
421      ACATCCTTTA AGTTTCTGGG GAAAAACGAA GATGGCAAAG GAAGATGTCC CTTTGACCCA
481      GCGCACAGCT ACACATCCGT CATGGTTGAT GGAGAACTTT ATTCAGGGAC GTCGTATAAT
541      TTTTTGGGAA GTGAACCCAT CATCTCCCGA AATTCTTCCC ACAGTCCTCT GAGGACGGAA
601      TACGCAATCC CTTGGCTGAA CGAGCCTAGT TTTGTGTTTG CCGACGTGAT TCGAAAAAGC
661      CCAGACAGCC CCGACGGCGA GGATGACAGG GTCTACTTCT TCTTCACAGA GGTGTCTGTG
721      GAGTACGAGT TTGTGTTCAG GGTGCTGATC CCACGGATAG CAAGAGTGTG CAAGGGGGAC
781      CAGGGCGGCC TGAGGACCTT GCAGAAGAAA TGGACCTCCT TCCTGAAAGC CCGACTCATC
841      TGCTCCCGGC CAGACAGCAG CTTGGTCTTC AATGTGCTGC GGGACGTCTT CGTGCTCAGG
901      TCCCCGGGCC TGAAGGTGCC TGTGTTCTAC GCACTCTTCA CCCCACAGCT GAACAACGTG
961      GGGCTGTCGG CAGTGTGCGC CTACAACCTG TCCATAGCCG AGGAGGTCTT CTCCCATGGG
1021     AAATACATGC AGAGCGCCAC GGTGGAGCAG TCCCACACCA AGTGGGTGCG CTACAACGGC
1081     CCGGTCCCCA AGCCGCGGCC TGGAGCGTGC ATCGACAGTG AGGCACGGGC AGCCAACTAC
1141     ACCAGCTCCT TGAATTTGCC AGACAAGACG CTGCAGTTCG TCAAAGACCA CCCTTTGATG
1201     GATGACTCGG TGACCCCAAT AGACAACAGG CCCAGGTTAA TCAAGAAAGA TGTGAACTAC
1261     ACCCAGATCG TGGTGGACCG GACCCAGGCC CTGGATGGGA CTGTCTATGA TGTCATGTTT
1321     GTCAGCACAG ACCGGGGAGC TCTGCACAAA GCCATCAGCC TCGAGCATGC TGTTCACATC
1381     ATCGAGGAGA CCCAGCTCTT CCAGGACTTT GAGCCGGTCC AGACCCTGCT GCTGTCTTCA
1441     AAGAAGGGCA GGAGGTTCGT CTATGCTGGC TCTAACTCGG GCGTGGTCCA GGCCCCTCTG
1501     GCCTTCTGTG GGAAGCACGG CACCTGCGAG GACTGTGTGC TGGCTCGGGA CCCCTACTGC
1561     GCCTGGAGCC CACCCACAGC GACGTGTGTG GCTCTGTACC AGACTGAGAG CCCCAGCAGG
1621     GGTTTGATTC AGGAGATGAG CGGCGATGCT TCTGTGTGCC CGGATAAAAG TAAAGGAAGT
1681     TACCGGCAGC ATTTTTTCAA GCACGGTGGC ACAGCGGAAC TGAAATGCTC CCAAAAGTCC
1741     AACCTGGCCC GGGTGTTTTG GAAGTTCCAG AATGGCGTGT TGAAGGCTGA GAGCCCCAAG
1801     TACGGTCTTA TGGGCAGAAA AAACTTGCTC ATCTTCAACT TATCAGAAGG AGACAGTGGG
1861     GTGTACCAGT GCCTGTCAGA GGAGAGGGTT AAGAACAAGA CGGTCTTCCA AGTAGTCGCC
1921     AAGCACGTCC TGGAAGTGAA GGTGGTTCCA AAGCCCGTGG TGGCCCCCAC CTTGTCAGTT
1981     GTTCAGACAG AAGGTAGTAG GATTGCCACC AAAGTGTTGG TGGCATCCAC CCAAGGGTCT
2041     TCTCCCCCGA CCCCAGCCGT GCAGGCCACC TCCTCCGGGG CCATCACCCT TCCTCCCAAG
2101     CCTGTGCCCA CCAGCACATC CTGTGAACCA AAGATTGTCA TCAACACGGT CCCCCAGCTC
2161     CACTCGGAGA AAACCATGTA TCTTAAGTCC AGCGATAACC GCCTCCTCAT GTCCCTCTTC
2221     CTCTTCTTCT TTGTTCTCTT CCTCTGCCTC TTTTTCTACA ACTGCTATAA GGGATACCTG
2281     CCCAGACAGT GCTTGAAATT CCGCTCGGCC CTGCTGATCG GGAAGAAGAA GCCCAAGTCA
2341     GATTTTTGTG ACCGTGAGCA GAGCCTGAAG GAGACGTTAG TGGAGCCAGG AAGCTTCTCC
2401     CAGCAGAATG GGGAGCACCC CAAGCCAGCC CTGGACACTG GCTATGAGAC TGAGCAAGAC
2461     ACCATCACCA GCAAAGTCCC CACGGACAGG GAGGACTCAC AGAGGATCGA CGACCTTTCC
2521     GCCAGGGACA AGCCCTTCGA CGTCAAGTGT GAGCTGAAGT TCGCGGACTC AGATGCAGAT
2581     GGAGACTGA

 

Quality Control:cDNA sequencing results is available upon request.