Rat SEMA4D Gene cDNA Clone / ORF Clone

SEMA4D cDNA Clone Datasheet

SEMA4D cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001170563.1
• cDNA Size: 2589
• Gene Synonym: Sema4d
• Source: Rat

SEMA4D cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 348 A/G and 1002 G/A not causing the amino acid variation.

SEMA4D cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SEMA4D cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SEMA4D cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGATGT GTGCCCCCGT CAGGGGGCTG TTCTTGGCCC TGGTGGCTGT GTGGAGGACC
61       GCGGTGGCAT TCGCCCCTGT GCCTCGGATC ACCTGGGAGC ACGGAGAGGT AGGTCTGGTG
121      AAGTTTCACG AGCCAGGCAT CTTTAACTAC TCTTCCTTGC TGATGAGTGA AGACAAAGAC
181      ACTCTGTATG TGGGTGCCCG GGAAGCTGTC TTTGCAGTGA ATGCGCTGGA CATCTCTGAG
241      AAGCAACATG AGGTATACTG GAAGGTCTCT GAAGACAAAA AATCCAAGTG CGCAGAGAAG
301      GGGAAATCAA AGCAGACGGA GTGCCTTAAC TACATCCGAG TGCTGCAGCC GCTTAGCAGC
361      ACTTCCCTCT ACGTGTGTGG GACCAATGCG TTCCAGCCCA CCTGTGACCA CCTGAACTTG
421      ACCTCTTTCA AGTTTCTGGG GAAAAGCGAA GATGGCAAAG GAAGATGCCC CTTCGACCCC
481      GCCCATAGCT ACACATCCGT CATGGTCGGG GGAGAGCTCT ACTCTGGGAC TTCATATAAT
541      TTCTTGGGCA GCGAACCCAT CATCTCTCGA AACTCTTCCC ACAGTCCCCT GAGGACAGAG
601      TACGCCATCC CTTGGCTAAA CGAGCCTAGC TTCGTCTTTG CTGACGTGAT CCACAAGAGC
661      CCAGATGGTA CAGAGGCTGA GGATGACAAG GTCTACTTCT TCTTTACGGA GGTGTCCGTG
721      GAGTACGAGT TCGTCTTCAA GTTGATGATC CCGCGAGTTG CCAGGGTGTG CAAGGGCGAC
781      CAGGGCGGCC TGCGGACTTT GCAAAAAAAG TGGACCTCCT TCCTAAAGGC CAGACTGATC
841      TGCTCCAGGC CAGACAGTGG CCTGGTCTTC AACATTCTTC AAGATGTGTT TGTGCTGAGG
901      GCCCCGGGCC TCAAGGAACC TGTGTTCTAT GCGGTCTTCA CCCCACAGCT GAACAACGTG
961      GGTCTGTCAG CGGTCTGTGC CTACACGCTG TCCACGGTGG AAGCCGTCTT CTCCCGAGGA
1021     AAGTACATGC AGAGTGCCAC AGTGGAGCAG TCTCACACCA AGTGGGTACG CTACAATGGC
1081     CCAGTGCCCA CTCCCCGGCC TGGAGCGTGT ATCGACAGTG AGGCCCGGGC AGCCAACTAC
1141     ACCAGCTCCT TGAATCTCCC AGACAAAACG CTGCAGTTTG TCAAAGACCA CCCTTTGATG
1201     GACGACTCGG TGACGCCAAT AGACAACAGG CCGAAACTGA TCAAAAAAGA TGTCAACTAC
1261     ACCCAGATAG TGGTAGACAG GACCCAGGCC CTGGATGGGA CCTTCTACGA CGTCATGTTC
1321     CTCAGCACAG ACCGGGGCGC TCTGCATAAA GCTGTCATCC TTGCAAAAGA GGTACACGTG
1381     GTTGAGGAGA CCCAACTCTT CCAGGACTTC GAACCGGTCC TGTCTCTGCT GCTATCATCA
1441     AAGAAGGGGA GGAAGTTTGT CTATGCTGGC TCCAACTCAG GAGTGGTCCA AGCTCCCCTG
1501     GCCTTCTGCG GAAAGCACAG TAGCTGTGAA GACTGTGTGC TAGCACGGGA CCCCTACTGC
1561     GCCTGGAGCC CAGCCATCAA GGCCTGTGTT ACCTTGCACC AGGCAGAGGG CTCTAGCAGG
1621     GGCTGGATTC AGGACATGAG TGGCGACACG TCCTCGTGCC TGGATAAGAG TAAAGAAAGT
1681     TTCCATCAGC ATTTTTTCAA GCACGGCGGC ACAGCAGAAC TCAAGTGTTT CCAAAAGTCC
1741     AACCTGGCCC GGGTGGTGTG GAAGTTCCAG AACGGCGAGT TGAAGGCTGT GAGTCCCAAG
1801     TATGGCTTTG TGGGCAGGAA GCACCTGCTC ATCTTTAACC TGTCAGACGG AGACAGCGGT
1861     GTGTACCAGT GCCTGTCAGA GGAAAGGGTC AGGAATAAAA CGGTCTCCCA GCTGCTCGCC
1921     AAGCACATCC TGGAAGTGAA AATGGTAGCT CGGATCCCCC CATCACCTAC CTCACAGACT
1981     GCTCAGACAG AAGGTAGTAG GATCACATCC AAAATGCCTG TGGCGTCTAC CCAGGGGTCC
2041     TCTCCCCCTA CCCCGGCTCT GTGGGCAACC TCCCCCAGGG CTGCCACCCT ACCTCCCAAG
2101     TCCTCCTCCA CCGGCACGTC CTGTGAACCA AAAATGGTCA TCAACACGGT CCCACAGCTC
2161     CACTCGGAGA AGACAGTGTA TCTCAAGTCC AGTGACAACC GCCTGCTCAT GTCTCTCCTC
2221     CTCTTCCTCT TTGTCCTCTT CCTCTGCCTC TTTTCCTACA ACTGCTACAA GGGCTACCTG
2281     CCCGGACAGT GCTTAAAGTT CCGCTCAGCC CTGCTGCTCG CAAAGAAAAA ACCCAAGTCA
2341     GAGTTCTCTG ACCTGGAGCA GAGTGTGAAG GAGACGCTGG TAGAACCTGG GAGCTTCTCG
2401     CAGCAGAACG GCGACCAGCC CAAGCCAGCC TTGGATACCG GCTATGAAAC CGAGCAGGAC
2461     ACTATCACCA GCAAGGTCCC CACCGATCGA GAGGACTCGC AACGTATCGA CGAGCTCTCC
2521     GCCAGGGACA AACCGTTTGA TGTCAAGTGT GAACTCAAGT TTGCAGACTC GGATGCCGAC
2581     GGGGACTGA

 

Quality Control:cDNA sequencing results is available upon request.