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Human SEMA3B Gene cDNA Clone (full-length ORF Clone)
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Human SEMA3B Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG12182-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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SEMA3B cDNA Clone Datasheet

 

SEMA3B cDNA Clone Product Information

Gene Bank Ref.ID: BC013975

cDNA Size:

2247

Gene Synonym:

SemA; SEMA5; SEMAA; semaV; LUCA-1;SEMA3B

Source: Human

SEMA3B cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens sema domain, immunoglobulin domain (Ig), short bas DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 2129 G/A resulting in the amino acid Arg substitution by His.

SEMA3B cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SEMA3B cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SEMA3B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGCGGG CCGGGGCTGC CGCCGTGATC CCGGGCCTGG CCCTGCTCTG GGCAGTGGGG
61       CTGGGGAGTG CCGCCCCCAG CCCCCCACGC CTTCGGCTCT CCTTCCAAGA GCTCCAGGCC
121      TGGCATGGTC TCCAGACTTT CAGCCTGGAG CGAACCTGCT GCTACCAGGC CTTGCTGGTG
181      GATGAGGAGC GTGGACGCCT GTTTGTGGGT GCCGAGAACC ATGTGGCCTC CCTCAACCTG
241      GACAACATCA GCAAGCGGGC CAAGAAGCTG GCCTGGCCGG CCCCTGTGGA ATGGCGAGAG
301      GAGTGCAACT GGGCAGGGAA GGACATTGGT ACTGAGTGCA TGAACTTCGT GAAGTTGCTG
361      CATGCCTACA ACCGCACCCA TTTGCTGGCC TGTGGCACGG GAGCCTTCCA CCCAACCTGT
421      GCCTTTGTGG AAGTGGGCCA CCGGGCAGAG GAGCCCGTCC TCCGGCTGGA CCCAGGAAGG
481      ATAGAGGATG GCAAGGGGAA GAGTCCTTAT GACCCCAGGC ATCGGGCTGC CTCCGTGCTG
541      GTGGGGGAGG AGCTATACTC AGGGGTGGCA GCAGACCTCA TGGGACGAGA CTTTACCATC
601      TTTCGCAGCC TAGGGCAACG TCCAAGTCTC CGAACAGAGC CACACGACTC CCGCTGGCTC
661      AATGAGCCCA AGTTTGTCAA GGTATTTTGG ATCCCGGAGA GCGAGAACCC AGACGACGAC
721      AAAATCTACT TCTTCTTTCG TGAGACGGCG GTAGAGGCGG CGCCGGCACT GGGACGCCTG
781      TCCGTGTCCC GCGTTGGCCA GATCTGCCGG AACGACGTGG GCGGCCAGCG CAGCCTGGTC
841      AACAAGTGGA CGACGTTCCT GAAGGCGCGG CTGGTGTGCT CGGTGCCCGG CGTCGAGGGC
901      GACACCCACT TCGATCAGCT CCAGGATGTG TTTCTGTTGT CCTCGCGGGA CCACCGGACC
961      CCGCTGCTCT ATGCCGTCTT CTCCACGTCC AGCATCTTCC AGGGCTCTGC GGTGTGCGTG
1021     TACAGCATGA ACGACGTGCG CCGGGCCTTC TTGGGACCCT TTGCACACAA GGAGGGGCCC
1081     ATGCACCAGT GGGTGTCATA CCAGGGTCGC GTCCCCTACC CGCGGCCAGG CATGTGCCCC
1141     AGCAAGACCT TTGGCACCTT CAGTTCCACC AAGGACTTCC CAGACGATGT CATCCAGTTT
1201     GCGCGGAACC ACCCCCTCAT GTACAACTCT GTCCTGCCCA CTGGGGGGCG CCCTCTTTTC
1261     CTACAAGTTG GAGCCAATTA CACCTTCACT CAAATTGCCG CGGACCGGGT TGCAGCCGCT
1321     GACGGACACT ATGACGTCCT CTTCATTGGC ACAGACGTTG GCACGGTGCT GAAGGTGATC
1381     TCGGTCCCCA AGGGCAGTAG GCCCAGCGCA GAGGGGCTGC TCCTGGAGGA GCTGCACGTG
1441     TTTGAGGACT CGGCCGCTGT CACCAGCATG CAAATTTCTT CCAAGAGGCA CCAGCTGTAC
1501     GTAGCCTCGC GGAGCGCGGT GGCCCAGATC GCGTTGCACC GCTGCGCTGC CCACGGCCGC
1561     GTCTGCACCG AATGCTGTCT GGCGCGTGAC CCCTACTGCG CCTGGGACGG GGTCGCGTGC
1621     ACGCGCTTCC AGCCCAGTGC CAAGAGGCGG TTCCGGCGGC AAGACGTAAG GAATGGCGAC
1681     CCCAGCACGT TGTGCTCCGG AGACTCGTCT CGTCCCGCGC TGCTGGAACA CAAGGTGTTC
1741     GGCGTGGAGG GCAGCAGCGC CTTTCTGGAG TGTGAGCCCC GCTCGCTGCA GGCGCGCGTG
1801     GAGTGGACTT TCCAGCGCGC AGGGGTGACA GCCCACACCC AGGTGCTGGC AGAGGAGCGC
1861     ACCGAGCGCA CCGCCCGGGG ACTACTGCTG CGCAGGCTGC GGCGCCGGGA CTCGGGCGTG
1921     TACTTGTGCG CCGCCGTCGA GCAGGGCTTT ACGCAACCGC TGCGTCGCCT GTCGCTGCAC
1981     GTGTTGAGTG CTACGCAGGC CGAACGACTG GCGCGGGCCG AGGAGGCTGC GCCCGCCGCG
2041     CCGCCGGGCC CCAAACTCTG GTACCGGGAC TTTCTGCAGC TGGTGGAGCC GGGCGGAGGT
2101     GGCAGCGCGA ACTCCCTGCG CATGTGCCAC CCGCAGCCTG CGCTGCAGTC ACTGCCCCTG
2161     GAGTCGCGGA GAAAGGGCCG TAACCGGAGG ACCCACGCCC CTGAGCCTCG CGCTGAGCGG
2221     GGGCCGCGCA GCGCAACGCA CTGGTGA

 

Quality Control:cDNA sequencing results is available upon request.

 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"