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> Gene Products > Mouse cDNA Clones > Mouse SEMA3A ORF Expression Clone, untagged Mouse SEMA3A ORF Expression Clone, untagged
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| MG50631-M-N |
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YES |  |
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* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
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SEMA3A cDNA Clone Datasheet
SEMA3A cDNA Clone Product Information
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Gene Bank Ref.ID: |
NM_009152.3 |
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cDNA Size: |
2319 |
| Gene Synonym: |
SemD; SEMA1; Semad; coll-1; Hsema-I; Sema3a |
| Source: | Mouse |
SEMA3A cDNA Clone Description
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cDNA Description: |
ORF Clone of Mus musculus sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A DNA. |
| Vector: | |
| Sequence Description: | Identical with the Gene Bank Ref. ID sequence except for the point mutation 1423 A/G resulting in the amino acid Ile substitution by Val. |
SEMA3A cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
SEMA3A cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
SEMA3A cDNA Clone Sequence and Quality Control
Sequence:
1 ATGGGCTGGT TCACTGGGAT TGCCTGTCTT TTCTGGGGTG TATTACTTAC AGCCAGAGCA 61 AACTATGCAA ACGGAAAGAA CAATGTGCCA AGACTGAAAT TATCGTACAA AGAAATGTTG 121 GAATCCAACA ATGTGATCAC TTTTAATGGC TTGGCCAACA GCTCCAGTTA CCACACCTTC 181 CTTCTGGATG AAGAACGGAG TAGACTATAT GTTGGAGCAA AAGATCATAT ATTTTCATTC 241 AACTTGGTGA ACATTAAAGA TTTTCAAAAG ATTGTGTGGC CAGTATCTTA CACAAGGAGA 301 GATGAATGCA AATGGGCTGG AAAAGATATC CTGAAAGAAT GTGCCAATTT CATCAAGGTC 361 CTGGAGGCTT ATAATCAGAC TCACTTGTAT GCCTGTGGAA CTGGGGCTTT CCATCCAATC 421 TGCACCTATA TTGAAGTTGG ACATCATCCT GAGGACAACA TTTTTAAGCT GCAGGACTCA 481 CATTTTGAAA ACGGTCGTGG GAAGAGCCCT TATGATCCCA AACTACTGAC TGCCTCTCTT 541 CTAATAGACG GTGAGTTGTA CTCTGGAACT GCTGCGGACT TCATGGGACG GGACTTCGCT 601 ATCTTCAGAA CACTGGGGCA CCATCACCCC ATCAGGACGG AGCAGCATGA CTCCCGGTGG 661 CTCAATGATC CTAGATTCAT CAGTGCCCAT CTCATCCCAG AGAGTGACAA CCCTGAAGAT 721 GACAAAGTAT ATTTTTTCTT CCGAGAAAAT GCAATAGACG GAGAACACTC TGGAAAAGCC 781 ACTCATGCTA GAATAGGTCA GATATGCAAG AATGACTTTG GTGGACACAG AAGTCTTGTG 841 AATAAATGGA CAACATTCCT AAAAGCACGC CTGATTTGCT CTGTGCCCGG TCCCAATGGC 901 ATTGACACCC ATTTTGATGA ATTGCAGGAT GTATTCCTAA TGAACTCTAA AGATCCTAAA 961 AATCCGATCG TCTATGGAGT GTTCACAACA TCAAGCAACA TCTTTAAGGG ATCTGCTGTG 1021 TGCATGTACA GCATGAGTGA TGTAAGAAGG GTGTTCCTTG GTCCATATGC TCACAGAGAT 1081 GGTCCCAACT ATCAGTGGGT GCCTTACCAA GGAAGAGTCC CTTATCCACG GCCAGGAACT 1141 TGTCCCAGTA AAACATTTGG CGGATTTGAC TCCACAAAGG ACCTTCCTGA TGATGTCATA 1201 ACTTTTGCAA GAAGTCATCC AGCCATGTAC AACCCAGTGT TTCCTATAAA TAATCGCCCG 1261 ATCATGATCA AAACAGATGT AAATTATCAG TTCACACAAA TTGTTGTAGA CCGAGTGGAT 1321 GCAGAAGATG GCCAGTATGA TGTTATGTTC ATCGGAACAG ATGTTGGAAC CGTTCTTAAA 1381 GTGGTTTCAG TCCCCAAGGA GACTTGGCAT GACCTAGAAG AAGTTCTTCT GGAAGAAATG 1441 ACCGTCTTCC GGGAACCAAC AACTATTTCG GCAATGGAGC TTTCTACTAA ACAGCAACAG 1501 CTGTACATTG GCTCAACTGC GGGAGTGGCA CAGCTTCCTC TACACCGCTG TGACATCTAT 1561 GGCAAAGCCT GTGCAGAATG CTGCCTCGCT CGGGACCCTT ACTGTGCCTG GGATGGGTCC 1621 TCATGCTCAC GCTATTTTCC TACTGCAAAG AGGCGCACAA GACGACAAGA TATAAGGAAT 1681 GGAGACCCAC TGACTCACTG CTCTGACTTG CAGCACCATG ATAATCATCA TGGGCCCAGC 1741 CTTGAAGAGA GAATCATCTA TGGAGTGGAA AACAGTAGTA CATTCTTGGA ATGCAGTCCG 1801 AAGTCACAGA GAGCCTTGGT ATATTGGCAA TTTCAGAGGA GAAATGAAGA TCGAAAAGAG 1861 GAGATCAGAA TGGGTGATCA TATCATCAGG ACAGAACAAG GGCTCCTGCT CCGTAGCCTG 1921 CAGAAGAAGG ATTCAGGCAA TTACCTGTGT CACGCTGTGG AACACGGATT CATGCAAACT 1981 CTTCTTAAGG TAACCCTGGA AGTCATTGAC ACAGAACATT TGGAAGAACT TCTTCATAAA 2041 GATGACGATG GAGATGGCTC TAAGATAAAA GAAATGTCGA GCAGCATGAC GCCCAGCCAG 2101 AAAGTCTGGT ACCGAGACTT CATGCAGCTC ATTAACCACC CCAACCTGAA CACGATGGAT 2161 GAGTTCTGTG AACAAGTGTG GAAAAGGGAC CGAAAGCAAC GCCGACAAAG GCCGGGGCAC 2221 TCTCAAGGGA GCAGCAACAA GTGGAAGCAC ATGCAAGAGA GCAAGAAAGG TAGAAACAGG 2281 AGGACCCACG AGTTTGAGCG GGCACCCAGA AGTGTCTAA
Quality Control:cDNA sequencing results is available upon request.
