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Mouse SEMA3A Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
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Mouse SEMA3A Gene cDNA Clone (full-length ORF Clone), expression ready, untagged

Catalog Vector (Price) Quantity In Stock Operation
MG50631-M-N
  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.

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SEMA3A cDNA Clone Datasheet

 

SEMA3A cDNA Clone Product Information

Gene Bank Ref.ID:

NM_009152.3

cDNA Size:

2319

Gene Synonym:

SemD; SEMA1; Semad; coll-1; Hsema-I; Sema3a

Source: Mouse

SEMA3A cDNA Clone Description

cDNA Description:

ORF Clone of Mus musculus sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A DNA.

Vector:

pCMV/hygro

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1423 A/G resulting in the amino acid Ile substitution by Val.

SEMA3A cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SEMA3A cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SEMA3A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCTGGT TCACTGGGAT TGCCTGTCTT TTCTGGGGTG TATTACTTAC AGCCAGAGCA
61       AACTATGCAA ACGGAAAGAA CAATGTGCCA AGACTGAAAT TATCGTACAA AGAAATGTTG
121      GAATCCAACA ATGTGATCAC TTTTAATGGC TTGGCCAACA GCTCCAGTTA CCACACCTTC
181      CTTCTGGATG AAGAACGGAG TAGACTATAT GTTGGAGCAA AAGATCATAT ATTTTCATTC
241      AACTTGGTGA ACATTAAAGA TTTTCAAAAG ATTGTGTGGC CAGTATCTTA CACAAGGAGA
301      GATGAATGCA AATGGGCTGG AAAAGATATC CTGAAAGAAT GTGCCAATTT CATCAAGGTC
361      CTGGAGGCTT ATAATCAGAC TCACTTGTAT GCCTGTGGAA CTGGGGCTTT CCATCCAATC
421      TGCACCTATA TTGAAGTTGG ACATCATCCT GAGGACAACA TTTTTAAGCT GCAGGACTCA
481      CATTTTGAAA ACGGTCGTGG GAAGAGCCCT TATGATCCCA AACTACTGAC TGCCTCTCTT
541      CTAATAGACG GTGAGTTGTA CTCTGGAACT GCTGCGGACT TCATGGGACG GGACTTCGCT
601      ATCTTCAGAA CACTGGGGCA CCATCACCCC ATCAGGACGG AGCAGCATGA CTCCCGGTGG
661      CTCAATGATC CTAGATTCAT CAGTGCCCAT CTCATCCCAG AGAGTGACAA CCCTGAAGAT
721      GACAAAGTAT ATTTTTTCTT CCGAGAAAAT GCAATAGACG GAGAACACTC TGGAAAAGCC
781      ACTCATGCTA GAATAGGTCA GATATGCAAG AATGACTTTG GTGGACACAG AAGTCTTGTG
841      AATAAATGGA CAACATTCCT AAAAGCACGC CTGATTTGCT CTGTGCCCGG TCCCAATGGC
901      ATTGACACCC ATTTTGATGA ATTGCAGGAT GTATTCCTAA TGAACTCTAA AGATCCTAAA
961      AATCCGATCG TCTATGGAGT GTTCACAACA TCAAGCAACA TCTTTAAGGG ATCTGCTGTG
1021     TGCATGTACA GCATGAGTGA TGTAAGAAGG GTGTTCCTTG GTCCATATGC TCACAGAGAT
1081     GGTCCCAACT ATCAGTGGGT GCCTTACCAA GGAAGAGTCC CTTATCCACG GCCAGGAACT
1141     TGTCCCAGTA AAACATTTGG CGGATTTGAC TCCACAAAGG ACCTTCCTGA TGATGTCATA
1201     ACTTTTGCAA GAAGTCATCC AGCCATGTAC AACCCAGTGT TTCCTATAAA TAATCGCCCG
1261     ATCATGATCA AAACAGATGT AAATTATCAG TTCACACAAA TTGTTGTAGA CCGAGTGGAT
1321     GCAGAAGATG GCCAGTATGA TGTTATGTTC ATCGGAACAG ATGTTGGAAC CGTTCTTAAA
1381     GTGGTTTCAG TCCCCAAGGA GACTTGGCAT GACCTAGAAG AAGTTCTTCT GGAAGAAATG
1441     ACCGTCTTCC GGGAACCAAC AACTATTTCG GCAATGGAGC TTTCTACTAA ACAGCAACAG
1501     CTGTACATTG GCTCAACTGC GGGAGTGGCA CAGCTTCCTC TACACCGCTG TGACATCTAT
1561     GGCAAAGCCT GTGCAGAATG CTGCCTCGCT CGGGACCCTT ACTGTGCCTG GGATGGGTCC
1621     TCATGCTCAC GCTATTTTCC TACTGCAAAG AGGCGCACAA GACGACAAGA TATAAGGAAT
1681     GGAGACCCAC TGACTCACTG CTCTGACTTG CAGCACCATG ATAATCATCA TGGGCCCAGC
1741     CTTGAAGAGA GAATCATCTA TGGAGTGGAA AACAGTAGTA CATTCTTGGA ATGCAGTCCG
1801     AAGTCACAGA GAGCCTTGGT ATATTGGCAA TTTCAGAGGA GAAATGAAGA TCGAAAAGAG
1861     GAGATCAGAA TGGGTGATCA TATCATCAGG ACAGAACAAG GGCTCCTGCT CCGTAGCCTG
1921     CAGAAGAAGG ATTCAGGCAA TTACCTGTGT CACGCTGTGG AACACGGATT CATGCAAACT
1981     CTTCTTAAGG TAACCCTGGA AGTCATTGAC ACAGAACATT TGGAAGAACT TCTTCATAAA
2041     GATGACGATG GAGATGGCTC TAAGATAAAA GAAATGTCGA GCAGCATGAC GCCCAGCCAG
2101     AAAGTCTGGT ACCGAGACTT CATGCAGCTC ATTAACCACC CCAACCTGAA CACGATGGAT
2161     GAGTTCTGTG AACAAGTGTG GAAAAGGGAC CGAAAGCAAC GCCGACAAAG GCCGGGGCAC
2221     TCTCAAGGGA GCAGCAACAA GTGGAAGCAC ATGCAAGAGA GCAAGAAAGG TAGAAACAGG
2281     AGGACCCACG AGTTTGAGCG GGCACCCAGA AGTGTCTAA

 

Quality Control:cDNA sequencing results is available upon request.


 

 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"