Human SELPLG Gene cDNA Clone / ORF Clone (Codon Optimized)

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13863-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

SELPLG cDNA Clone Datasheet

SELPLG cDNA Clone Product Information

cDNA Size:	1287
Gene Synonym:	CLA; CD162; PSGL1; PSGL-1;SELPLG
Source:	Human

SELPLG cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens selectin P ligand DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with NP_001193538.1.

SELPLG cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SELPLG cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SELPLG cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCAGTGG GGGCCAGTGG TCTAGAAGGA GATAAGATGG CTGGTGCCAT GCCTCTGCAA
61       CTCCTCCTGT TGCTGATCCT ACTGGGCCCT GGCAACAGCT TGCAGCTGTG GGACACCTGG
121      GCAGATGAAG CCGAGAAAGC CTTGGGTCCC CTGCTTGCCC GGGACCGGAG ACAGGCCACC
181      GAATATGAGT ACCTAGATTA TGATTTCCTG CCAGAAACGG AGCCTCCAGA AATGCTGAGG
241      AACAGCACTG ACACCACTCC TCTGACTGGG CCTGGAACCC CTGAGTCTAC CACTGTGGAG
301      CCTGCTGCAA GGCGTTCTAC TGGCCTGGAT GCAGGAGGGG CAGTCACAGA GCTGACCACG
361      GAGCTGGCCA ACATGGGGAA CCTGTCCACG GATTCAGCAG CTATGGAGAT ACAGACGACC
421      CAGCCTGCCG CAACCGAGGC CCAGACCACG CAGCCCGTGC CCACGGAGGC GCAGACGACG
481      CCCCTGGCCG CCACCGAAGC CCAGACAACT CGACTGACGG CCACGGAGGC ACAGACCACT
541      CCACTGGCAG CCACAGAGGC ACAGACCACT CCACCAGCAG CCACGGAAGC ACAGACCACT
601      CAACCCACAG GCCTGGAGGC ACAGACCACT GCACCAGCAG CCATGGAGGC ACAGACCACT
661      GCACCAGCAG CCATGGAAGC ACAGACCACT CCACCAGCAG CCATGGAGGC ACAGACCACT
721      CAAACCACAG CCATGGAGGC ACAGACCACT GCACCAGAAG CCACGGAGGC ACAGACCACT
781      CAACCCACAG CCACGGAGGC ACAGACCACT CCACTGGCAG CCATGGAGGC CCTGTCCACA
841      GAACCCAGTG CCACAGAGGC CCTGTCCATG GAACCTACTA CCAAAAGAGG TCTGTTCATA
901      CCCTTTTCTG TGTCCTCTGT TACTCACAAG GGCATTCCCA TGGCAGCCAG CAATTTGTCC
961      GTCAACTACC CAGTGGGGGC CCCAGACCAC ATCTCTGTGA AGCAGTGCCT GCTGGCCATC
1021     CTAATCTTGG CGCTGGTGGC CACTATCTTC TTCGTGTGCA CTGTGGTGCT GGCGGTCCGC
1081     CTCTCCCGCA AGGGCCACAT GTACCCCGTG CGTAATTACT CCCCCACCGA GATGGTCTGC
1141     ATCTCATCCC TGTTGCCTGA TGGGGGTGAG GGGCCCTCTG CCACAGCCAA TGGGGGCCTG
1201     TCCAAGGCCA AGAGCCCGGG CCTGACGCCA GAGCCCAGGG AGGACCGTGA GGGGGATGAC
1261     CTCACCCTGC ACAGCTTCCT CCCTTAG

Quality Control:cDNA sequencing results is available upon request.