SELP / selectin P / P-selectin Protein

Selectin P (granule membrane protein 140kDa, antigen CD62) Protein Datasheet

SELP Protein Product Information

• Synonym: RP1-86F14.2, CD62, CD62P, GMP140, GRMP, LECAM3, PADGEM, PSEL
• Protein Construction: A DNA sequence encoding the human SELP (AAN06828.1) extracellular domain (Met 1- Ala 771) was fused with the Fc region of human IgG₁ at the C-terminus.
• Source: Human
• Expression Host: Human Cells

SELP Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Trp 42
• Molecular Mass: The recombinant human SELP/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 971 amino acids and has a predicted molecular mass of 107 kDa. The apparent molecular mass of the reduced monomer is approximately 130-150 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: SELP protein

SELP Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SELP Protein Description

P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interacton of activated endothelial cells or platelets with leukocytes.

References

1. Johnson-Tidey RR. et al., 1994, Am J Pathol. 144 (5): 952-61.
2. Walcheck B. et al., 1996, J Clin Invest. 98 (5): 1081-7.
3. Foreman KE. et al., 1994, J Clin Invest. 94 (3): 1147-55.