Cynomolgus monkey SELP Gene cDNA Clone / ORF Clone

SELP cDNA Clone Datasheet

SELP cDNA Clone Product Information

• cDNA Size: 2493
• Gene Synonym: SELP
• Source: Cynomolgus monkey

SELP cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) selectin P (granule membrane protein 140kDa, antigen CD62) DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 123 A/G, 210 T/C and 1485 T/C not causing the amino acid variation.
  Please check the sequence information before order.

SELP cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SELP cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SELP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCAGCT GCCAAAAAGC CATCTTGTAT CAGAGATTCC GGAGAGTGGT CTTTGGAATT
61       GCCCGACTCC TTTGCTTCAG TGCCCTGATC TCTGAACTAA CAAACCAGAA AGAAGTGGCA
121      GCGTGGACTT ATCATTACAG CACAAAAGCA TACTCATGGA ATACTTCCCG CAAATACTGC
181      CAGAATCGCT ACACAGACTT AGTGGCCATC CAGAATAAAA AGGAAATTGA TTACCTCAAT
241      GAGGTCCTAC CCTACTACAG CACCTACTAC TGGATTGGGA TCCGAAAGAG CAATAAGACA
301      TGGACCTGGG TGGGAACCAA AAAGGCTCTC ACTAAAGAGG CTGAGAACTG GGCTGATAAT
361      GAACCTAACA ACAAAAGGAA CAACGAGGAC TGCGTGGAGA TCTACATCAA GAGTCCGTCA
421      GCCCCTGGCA AGTGGAATGA TGAGCACTGC TTGAAGAAAA AGCACGCATT GTGTTACACA
481      GCCTCCTGCC AGGACATGTC CTGCAGCAAA CAAGGAGAGT GCCTCGAGAC CATCGGGAAC
541      TACACCTGCT CCTGTTACCC TGGATTCTAT GGGCCAGAAT GTGAATATGT GACAGAGTGT
601      GGAGAACTTG AGCTCCCTCA ACACGTGCTT ATGAACTGCA GCCACCCTCT GGGAAACTTC
661      TCTTTTAACT CGCAGTGCAG CTTCCACTGT GCTGACGGGT ACCAAGTAAA TGGGCCCAGC
721      AAGCTGGAAT GCTTGGCTTC TGGAATCTGG ACACATAAGC CTCCGCAGTG TTTAGCTGCC
781      CAGTGCCCAC CCCTGAAGAT TCCTGAAAGA GGAAACATGA CCTGCCTTCA TTCTGCAAAA
841      GCATTCCAGC ATCAGTCTAG CTGCGGCTTC AGTTGTGAAG AGGGATTTGT ATTAGTTGGA
901      CCAGAAGTGG TGCAATGCAC AGCCTCGGGG GTATGGACAG CCCCAGCCCC AGTGTGTAAA
961      GCTGTGCAGT GTCAGCACCT GGAAGCCCCC AGCGAAGGAA CCATGGACTG TATTCATCCA
1021     CTCGCTGCTT TTGCATATGG CTCCAACTGT AAATTTGAGT GCCAGCTCGG CTACAGAGTG
1081     AGGGGCTTGG ACACGCTCCG TTGCATTGGC TCTGGACACT GGTCTGCACC CTTGCCAACC
1141     TGTGAGGCTA TTTCGTGTGA GCCGCTGGAG AGTCCTGTCC ACGGAAGCAT GGATTGCTCT
1201     CCATCCTTGA GAGCGTTTCA GTATGATACC AACTGTAGCT TCCGCTGTGC TGAAGGTTTC
1261     ATGCTGAGAG GAGCTGATAT AGTTCGGTGT GATAACTTGG GACAGTGGAC AGCGCCAGCC
1321     CCAGTCTGTC AAGCTTTGCA GTGCCAGGAT CTCCCAGTTC CAAATGAGGC TCAGGTGAAC
1381     TGCTCCCACC CCTTTGGTGC CTTTAGGTAC CAGTCAGTCT GCAGCTTCAC CTGCAATGAA
1441     GACTTACTCT TGGTGGGAGC GAGTGTGCTA CAGTGCTTGG CTACCGGAAA CTGGAATTCT
1501     GTTCCTCCAG AATGCCAAGC CATTCCCTGC ACACCTTTGC TAAGCCCTCA GAATGGAACA
1561     ATGACCTGTG TCCAGCCTCT TGGAAGTTCC AGTTATAAAT CCACCTGTCA CTTCATCTGT
1621     GATGAGGGAT TTTCTTTGTC TGGACCAGAA AGATTGGATT GTACTCGATC GGGACGCTGG
1681     ACAGACTCCC CACCAACGTG TGAAGCCATC AAGTGCCCAG AACTCTTCGC CCCAGAGCAG
1741     GGCAGCCTGG ATTGTTCTGA CACTCATGGA GAATTCAATG TTGGCTCCAC CTGCCATTTC
1801     TCTTGTAACA AGGGCTTTAA GCTGGAGGGG CCCAATAATG TGAAATGCAC AACTTCTGGA
1861     AGATGGTCAG CTACTCCACC AGCCTGCAAA GGCATAGCAT CACTTCCTAG TCCAGGGGTG
1921     CAATGCCCAG CCCTCACCAC ACCTGGGCAG GGAACCATGC ACTGTAGGCA TCATCCGGGA
1981     ACCTTTGGTT TTAATACCAC TTGTTACTTT GGCTGCAACG CTGGATTCAC ACTCACAGGA
2041     GACAGCATTC TCAGCTGCAG ACCTTCAGGA CAATGGACAG CAGTAACTCC AACATGCAGA
2101     GCTGTGAAAT GCCCAGAACT ACATGTTAAT AAGCCAATAG TGATGAACTG CTCCAACCTC
2161     TGGGGAAACT TCAGTTATGG ATCAATCTGC TCTTTCCATT GTCTAGAGGG CCAGTTACTT
2221     AATGGCTCAG CACAAACAGC ATGCCAGGAG AATGGCCACT GGTCAACTAC CGTGCCAACC
2281     TGCCAAGCAG GACCACTGAC TATTCAGGAA GCCCTGACTT ACTTTGGTGG AGCGGTGGCT
2341     TCTACAACAG GTCTGATAAT GGGTGGGACG CTCCTGGCTT TGCTAAGAAA GCGTTTCAGA
2401     CAAAAAGATG ATGGGAAATG CCCCTTGAAT CCTCACAGCC ACCTAGGAAC ATATGGAGTT
2461     TTTACAAATG CTGCATTTGA CCCGAGTTCT TAA

 

Quality Control:cDNA sequencing results is available upon request.