Cynomolgus monkey SELL Gene cDNA Clone / ORF Clone

SELL cDNA Clone Datasheet

SELL cDNA Clone Product Information

• cDNA Size: 1119
• Gene Synonym: SELL
• Source: Cynomolgus monkey

SELL cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) selectin L DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 439 T/C resulting in the amino acid Cys substitution by Arg.
  Please check the sequence information before order.

SELL cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SELL cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SELL cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATATTTC CACGGAAATG TCAGAGTACC CAGAGGGACT TATGGAACAT CTTCAAGTTG
61       TGGGGGTGGA CAATGCTCTG TTGTGATTTC CTGGCACATC ATGGAACCGA CTGCTGGACT
121      TACCATTATT CTGAAAATCC CATGAACTGG CAAAAGGCTA GAAGATTCTG CCGAGAGAAT
181      TACACAGATT TAGTTGCCAT ACAAAACAAG GCAGAAATTG AGTATCTGGA GAAGACTCTG
241      CCTTTCAGTC CTTCTTACTA CTGGATAGGA ATCCGGAAGA TAGGAGGCAT ATGGACGTGG
301      GTGGGAACCA ACAAATCTCT CACTCAAGAA GCAGAGAACT GGGGAGATGG GGAGCCCAAC
361      AACAAGAAAA ACAAGGAGGA CTGCGTGGAG ATCTATATCA AGAGAAAGAA AGATGCGGGC
421      AAATGGAATG ATGACGCCCG CCACAAACCA AAGGCAGCCC TCTGTTACAC AGCTTCTTGC
481      CAGCCCTGGT CATGCAGTGG CCATGGAGAA TGTGTAGAAA TCATCAATAA TTACACCTGC
541      AACTGTGATG TGGGGTACTA TGGGCCCCAG TGTCAGTTTG TGATTCAGTG TGAGCCTTTG
601      GAGCCCCCGA AGCTGGGTAC CATGGACTGT ACTCACCCTT TGGGAGACTT CAGCTTCAGC
661      TCACAGTGTG CCTTCAACTG CTCTGAGGGA ACAAACTTAA CTGGGATTGA AGAAACCACT
721      TGTGGACCAT TTGGAAACTG GTCATCTCCA GAACCAACCT GTCAAGTGAT TCAGTGTGAG
781      CCTCTATCAG CACCAGATTT GGGGATCATG AACTGTAGTC ACCCCCTGGC CAGCTTCAGC
841      TTTTCCTCTG CATGTACCTT CAGCTGCTCA GAAGGAACTG AGCTAATTGG GGAGAAGAAA
901      ACCATTTGTG AGTCATCTGG AATCTGGTCA AATCCTAATC CAATATGTCA AAAATTGGAC
961      AGAAGTTTCT CAATGATTAA GGAGGGTGAT TATAACCCCC TCTTCATTCC AGTGGCAGTC
1021     ATGGTTACTG CATTCTCTGG GTTGGCATTT ATCATTTGGC TGGCAAGGAG ATTAAAAAAA
1081     GGCAAGAAAT CCAAGAAAAG TATGGATGAC CCATATTAA

 

Quality Control:cDNA sequencing results is available upon request.